An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain

• Grant number: 16/08176-9
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: May 01, 2017
• End date: June 30, 2020
• Field of knowledge: Health Sciences - Collective Health - Epidemiology
• Principal Investigator: Carlos Eduardo de Almeida
• Grantee: Carlos Eduardo de Almeida
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: André Victor Lucci Freitas ; Anete Pereira de Souza ; Danilo Ciccone Miguel ; Elaine Folly Ramos ; João Aristeu da Rosa ; Myriam Harry ; Patricia Jacqueline Thyssen ; Paulo Sergio D' Andrea
• Associated scholarship(s): 18/20814-6 - Eco-epidemiological aspects of Chagas Disease and Trypanosoma cruzi genotyping of vectors from chagasic outbreak sites in Rio Grande do Norte, BP.IC; 17/21359-8 - Integrated molecular approaches to elucidate the ecological associations of Triatoma brasiliensis: food sources, microbiota and diversity of Trypanosoma cruzi, BP.DR; 18/21214-2 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, BP.TT; + associated scholarships 18/20053-5 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, BP.TT; 17/19420-0 - Molecular tracking of Trypanosoma cruzi from Chagas disease vectors in an outbreak area in Rio Grande do Norte, Brazil, BP.PD; 18/08795-6 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, BP.TT; 18/04098-9 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, BP.TT; 18/04594-6 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, BP.DD; 18/04205-0 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, BP.MS; 17/09088-9 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, BP.JP - associated scholarships

Abstract

In semi-arid areas of Brazil, Triatoma brasiliensis is the most important Chagas disease vector, with potential to elucidate aspects of other elements of eco-epidemiologic chain. Therefore, we will use an integrative method applied to T. brasiliensis specimens collected in sites with records of high domiciliary infestation pressure and chagasic outbreaks in the State of Rio Grande do Norte, Brazil. The re-infestation foci (via population genetics) and demographic aspects of T. brasiliensis populations under control activities (via Approximate Bayesian Computation - ABC) will be elucidated by using DNA extracted from legs of insects via two molecular markers: cytochrome b (cytb) fragments and single-nucleotide polymorphisms (SNPs-ddRAD/3dRAD), combined with the geometric morphometrics by using the heads and wings. By using next-generation sequencing (NGS) we will explore genes associated to domiciliation process by analyzing transcriptomes from antennae and rostrum. The Trypanosoma cruzi genotyping, T. brasiliensis feeding sources and T. cruzi reservoirs will be identifying by using NGS to the blood meal ingested by the insect through metagenomics/metabarcoding. The landscape genetics will be applied by combining morphological and genotypic information via ecological and spatial data, aiming to detect corridors for population dispersion of insects that invade homes. This project counts with an innovative and synergic model-approach to determine the vector genetic structure, the genetic base related to domiciliation process, the parasite lineages, T. cruzi reservoir and factor that make possible the vector dispersion to domiciles. Finally, an integrated analysis of results will be converted to a comprehensive way to be passed to those involved in the vector control. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DALE, CAROLINA; ALMEIDAZ, CARLOS EDUARDO; MENDONCA, VAGNER JOSE; OLIVERIA, JADER; DA ROSA, JOAO ARISTEU; GALVDOS, CLEBER; COSTA, JANE. An updated and illustrated dichotomous key for the Chagas disease vectors of Triatoma brasiliensis species complex and their epidemiologic importance. ZOOKEYS, n. 805, p. 33-43, DEC 11 2018. (16/08176-9, 17/09088-9)

LILIOSO, MAURICIO; PIRES-SILVA, DAYANE; MASCARENHAS FONTES, FERNANDA VON HERTWIG; OLIVEIRA, JADER; DA ROSA, JOAO ARISTEU; VILELA, ROBERTO V.; FOLLY-RAMOS, ELAINE; ALMEIDA, CARLOS EDUARDO. Triatoma petrocchiae (Hemiptera, Reduviidae, Triatominae): A Chagas disease vector of T. brasiliensis species complex associated to reptiles. INFECTION GENETICS AND EVOLUTION, v. 82, AUG 2020. (16/08176-9, 17/21359-8)

OLIVEIRA, JADER; MARCET, PAULA L.; TAKIYA, DANIELA M.; MENDONCA, VAGNER J.; BELINTANI, TIAGO; BARGUES, MARIA D.; MATEO, LUCIA; CHAGAS, VIVIAN; FOLLY-RAMOS, ELAINE; CORDEIRO-ESTRELA, PEDRO; et al. Combined phylogenetic and morphometric information to delimit and unify the Triatoma brasiliensis species complex and the Brasiliensis subcomplex. Acta Tropica, v. 170, p. 140-148, JUN 2017. (13/08826-5, 16/08176-9)

LIMA-OLIVEIRA, TARCIANNE M.; FONTES, FERNANDA VON HERTWIG M.; LILIOSO, MAURICIO; PIRES-SILVA, DAYANE; GERALDES TEIXEIRA, MARTA MARIA; VERGARA MEZA, JOSE GABRIEL; HARRY, MYRIAM; FILEE, JONATHAN; COSTA, JANE; VALENCA-BARBOSA, CAROLINA; et al. Molecular eco-epidemiology on the sympatric Chagas disease vectors Triatoma brasiliensis and Triatoma petrocchiae: Ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping. Acta Tropica, v. 201, JAN 2020. (17/50329-0, 16/08176-9, 17/21359-8, 17/09088-9)

DE OLIVEIRA, JADER; CHABOLI ALEVI, KAIO CESAR; ALMEIDA, CARLOS EDUARDO; MENDONCA, VAGNER JOSE; COSTA, JANE; DA ROSA, JOAO ARISTEU. Triatoma brasiliensis species complex: characterization of the external female genitalia. JOURNAL OF VECTOR ECOLOGY, v. 45, n. 1, p. 57-68, JUN 2020. (16/08176-9, 13/08826-5)

MONSALVE-LARA, JACKELINE; LILIOSO, MAURICIO; VALENCA-BARBOSA, CAROLINA; THYSSEN, PATRICIA J.; MIGUEL, DANILO C.; LIMEIRA, CLEANNE; GADELHA, FERNANDA R.; FONTES, FERNANDA V. H. M.; PIRES-SILVA, DAYANE; DORNAK, L. LYNNETTE; et al. The risk of oral transmission in an area of a Chagas disease outbreak in the Brazilian northeast evaluated through entomological, socioeconomic and schooling indicators. Acta Tropica, v. 215, MAR 2021. (16/08176-9)

JANE COSTA; CAROLINA DALE; CLEBER GALVÃO; CARLOS EDUARDO ALMEIDA; JEAN PIERRE DUJARDIN. Do the new triatomine species pose new challenges or strategies for monitoring Chagas disease? An overview from 1979-2021. Memórias do Instituto Oswaldo Cruz, v. 116, 2021-05-31. (16/08176-9)

DALE, CAROLINA; ALMEIDAZ, CARLOS EDUARDO; MENDONCA, VAGNER JOSE; OLIVERIA, JADER; DA ROSA, JOAO ARISTEU; GALVDOS, CLEBER; COSTA, JANE. An updated and illustrated dichotomous key for the Chagas disease vectors of Triatoma brasiliensis species complex and their epidemiologic importance. ZOOKEYS, v. N/A, n. 805, p. 11-pg., 2018-12-11. (16/08176-9, 17/09088-9)

LILIOSO, MAURICIO; FOLLY-RAMOS, ELAINE; ROCHA, FABIANA LOPES; RABINOVICH, JORGE; CAPDEVIELLE-DULAC, CLAIRE; HARRY, MYRIAM; MARCET, PAULA L.; COSTA, JANE; ALMEIDA, CARLOS EDUARDO. High Triatoma brasiliensis Densities and Trypanosoma cruzi Prevalence in Domestic and Peridomestic Habitats in the State of Rio Grande do Norte, Brazil: The Source for Chagas Disease Outbreaks?. American Journal of Tropical Medicine and Hygiene, v. 96, n. 6, p. 1456-1459, 2017. (16/08176-9)

LILIOSO, MAURICIO; REIGADA, CAROLINA; PIRES-SILVA, DAYANE; FONTES, FERNANDA VON H. M.; LIMEIRA, CLEANNE; MONSALVE-LARA, JACKELINE; FOLLY-RAMOS, ELAINE; HARRY, MYRIAM; COSTA, JANE; ALMEIDA, CARLOS EDUARDO. Dynamics of food sources, ecotypic distribution and Trypanosoma cruzi infection in Triatoma brasiliensis from the northeast of Brazil. PLoS Neglected Tropical Diseases, v. 14, n. 9, SEP 2020. (17/50329-0, 16/08176-9, 17/21359-8)

KAMIMURA, EDGARD H.; VIANA, MARIA CAROLINA; LILIOSO, MAURICIO; FONTES, FERNANDA H. M.; PIRES-SILVA, DAYANE; VALENCA-BARBOSA, CAROLINA; CARBAJAL-DE-LA-FUENTE, ANA L.; FOLLY-RAMOS, ELAINE; SOLFERIN, VERA N.; THYSSEN, PATRICIA J.; et al. Drivers of molecular and morphometric variation in Triatoma brasiliensis (Hemiptera: Triatominae): the resolution of geometric morphometrics for populational structuring on a microgeographical scale. PARASITES & VECTORS, v. 13, n. 1, p. 15-pg., 2020-09-07. (17/19420-0, 16/08176-9, 17/21359-8, 17/09088-9, 18/04594-6, 17/50329-0, 18/04205-0)

VALENCA-BARBOSA, CAROLINA; FINAMORE-ARAUJO, PAULA; MOREIRA, OTACILIO C.; VERGARA-MEZA, JOSE GABRIEL; NIZ ALVAREZ, MARCUS VINICIUS; NASCIMENTO, JULIANA R.; BORGES-VELOSO, ANDRE; VIANA, MARIA CAROLINA; LILIOSO, MAURICIO; MIGUEL, DANILO CICCONE; et al. Genotypic Trypanosoma cruzi distribution and parasite load differ ecotypically and according to parasite genotypes in k from endemic and outbreak areas in Northeastern Brazil. Acta Tropica, v. 222, OCT 2021. (16/08176-9)

FILEE, JONATHAN; MERLE, MARIE; BASTIDE, HELOISE; MOUGEL, FLORENCE; BERENGER, JEAN-MICHEL; FOLLY-RAMOS, ELAINE; ALMEIDA, CARLOS EDUARDO; HARRY, MYRIAM. hylogenomics for Chagas Disease Vectors of the Rhodnius Genus (Hemiptera, Triatominae): What We Learn From Mito-Nuclear Conflicts and Recommendation. FRONTIERS IN ECOLOGY AND EVOLUTION, v. 9, JAN 12 2022. (16/08176-9, 17/50329-0)

DAFLON-TEIXEIRA, NATALIA FARIA; COUTINHO, CAROLINA; GOMES, TAIS FERREIRA; TOMA, HELENA KEIKO; DUARTE, ROSEMERE; BOIA, MARCIO NEVES; CARVALHO-COSTA, FILIPE ANIBAL; ALMEIDA, CARLOS EDUARDO; LIMA, MARLI MARIA. Multiple Approaches to Address Potential Risk Factors of Chagas Disease Transmission in Northeastern Brazil. American Journal of Tropical Medicine and Hygiene, v. 100, n. 2, p. 296-302, 2019. (16/08176-9)

MARCHANT, AXELLE; MOUGEL, FLORENCE; JACQUIN-JOLY, EMMANUELLE; ALMEIDA, CARLOS E.; BLANCHET, DENIS; BERENGER, JEAN-MICHEL; DA ROSA, JOAO A.; HARRY, MYRIAM. Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease. FRONTIERS IN ECOLOGY AND EVOLUTION, v. 9, OCT 6 2021. (16/08176-9, 17/50329-0)