Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

Author(s)

Sipauba-Tavares, L. H. ^[1] ; Millan, R. N. ^{[1, 2]} ; Berchielli-Morais, F. A. ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Ctr Aquicultura, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Programa Posgrad Microbiol Agr, BR-14884900 Jaboticabal, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Biology; v. 73, n. 3, p. 585-591, AUG 2013.
• Web of Science Citations: 0

Abstract

Growth rate and medium parameters between two bench scale volumes (13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of medium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 10(5) cells. mL(-1) on the 12th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water culture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions. (AU)

• FAPESP's process: 09/51946-6 - Astaxanthin production from microalgae Haematococcus pluvialis: implementation and evaluation
• Grantee: Lúcia Helena Sipaúba Tavares
• Support Opportunities: Regular Research Grants