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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PRP8 intein in cryptic species of Histoplasma capsulatum: Evolution and phylogeny

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Author(s):
Theodoro, Raquel Cordeiro [1] ; Scheel, Christina M. [2] ; Brandt, Mary E. [2] ; Kasuga, Takao [3] ; Bagagli, Eduardo
Total Authors: 5
Affiliation:
[1] Univ Fed Rio Grande do Norte, BR-59072970 Natal, RN - Brazil
[2] Ctr Dis Control & Prevent CDC, Mycot Dis Branch, Atlanta, GA - USA
[3] USDA ARS, Davis, CA - USA
Total Affiliations: 3
Document type: Journal article
Source: INFECTION GENETICS AND EVOLUTION; v. 18, p. 174-182, AUG 2013.
Web of Science Citations: 8
Abstract

The PRP8 intein is the most widespread intein among the Kingdom Fungi. This genetic element occurs within the prp8 gene, and is transcribed and translated simultaneously with the gene. After translation, the intein excises itself from the Prp8 protein by an autocatalytic splicing reaction, subsequently joining the N and C terminals of the host protein, which retains its functional conformation. Besides the splicing domain, some PRP8 inteins also have a homing endonuclease (HE) domain which, if functional, makes the intein a mobile element capable of becoming fixed in a population. This work aimed to study (1) The occurrence of this intein in Histoplasma capsulatum isolates (n = 99) belonging to different cryptic species collected in diverse geographical locations, and (2) The functionality of the endonuclease domains of H. capsulatum PRP8 inteins and their phylogenetic relationship among the cryptic species. Our results suggest that the PRP8 intein is fixed in H. capsulatum populations and that an admixture or a probable ancestral polymorphism of the PRP8 intein sequences is responsible for the apparent paraphyletic pattern of the LAmA clade which, in the intein phylogeny, also encompasses sequences from LAmB isolates. The PRP8 intein sequences clearly separate the different cryptic species, and may serve as an additional molecular typing tool, as previously proposed for other fungi genus, such as Oyptococcus and Paracoccidioides. (C) 2013 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/08839-1 - PRP8 intein in pathogenic fungi: evolutionary aspects and therapeutic potential
Grantee:Raquel Cordeiro Theodoro
Support Opportunities: Scholarships in Brazil - Post-Doctoral