| Full text | |
| Author(s): |
Vidal, Benedicto de Campos
[1]
Total Authors: 1
|
| Affiliation: | [1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-01308386 Campinas, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | ACTA HISTOCHEMICA; v. 115, n. 7, p. 686-691, 2013. |
| Web of Science Citations: | 8 |
| Abstract | |
Polarized Fourier transform-infrared (FT-IR) was used to compare the orientation of vibrational chemical groups of bovine tendon collagen bundles (CBs) to that of nylon 6, a simpler polyamide model, in terms of linear dichroism (LD). Subtraction of spectral profiles identified the most significant differences regarding the amide regions. At 1630 cm(-1), the CBs displayed higher peak areas and absorbance when positioned perpendicularly (A(perpendicular to)) to the plane of polarized light, in comparison with nylon 6. In contrast, at the 1526 cm(-1) amide II spectral region the inverse occurred. In the amide III region (1232 cm(-1)), the LD was positive and higher for CBs. Dichroic ratios (DR=A(parallel to)/A(perpendicular to)) calculated from the average of ten measured spectra for CBs and nylon 6 revealed that the values for CBs were <1.0 in the 3298-1655 cm(-1) wavenumber range and >1.0 in the 1536-1234 cm(-1) wavenumber range. From 1535 to 1120 cm(-1), nylon 6 displayed DR values higher than those of CBs. The LD band integrated areas were higher in CBs than in nylon 6. The LD differences between CBs and nylon 6 are probably due to a more complex chemical composition and supramolecular oriented architecture in CBs in comparison to nylon 6. (C) 2013 Elsevier GmbH. All rights reserved. (AU) | |
| FAPESP's process: | 03/04597-0 - Molecular order and supramolecular organization of collagen bundles: differences in functionality, as a supposed effect of expression of pathogenesis, and methodological contribution |
| Grantee: | Benedicto de Campos Vidal |
| Support Opportunities: | Regular Research Grants |