| Full text | |
| Author(s): |
L. Sbragia
[1]
;
A.C.C. Nassr
[2]
;
F.L.L. Gonçalves
[3]
;
A.F. Schmidt
[4]
;
C.C. Zuliani
[5]
;
P.V. Garcia
[6]
;
R.M. Gallindo
[1]
;
L.A.V. Pereira
[6]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Div Cirurgia Pediat, Dept Cirurgia & Anat, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Hidrobiol, Ctr Ciencias Biol & Saude, BR-13560 Sao Carlos, SP - Brazil
[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia - Brasil
[4] Cincinnati Childrens Hosp Med Ctr, Pediat House Off, Cincinnati, OH 45229 - USA
[5] Univ Estadual Campinas, Fac Ciencias Med, Dept Clin Med, Campinas, SP - Brazil
[6] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Histol & Embriol, Campinas, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Medical and Biological Research; v. 47, n. 2, p. 171-178, 2014-02-00. |
| Abstract | |
Changes in vascular endothelial growth factor (VEGF) in pulmonary vessels have been described in congenital diaphragmatic hernia (CDH) and may contribute to the development of pulmonary hypoplasia and hypertension; however, how the expression of VEGF receptors changes during fetal lung development in CDH is not understood. The aim of this study was to compare morphological evolution with expression of VEGF receptors, VEGFR1 (Flt-1) and VEGFR2 (Flk-1), in pseudoglandular, canalicular, and saccular stages of lung development in normal rat fetuses and in fetuses with CDH. Pregnant rats were divided into four groups (n=20 fetuses each) of four different gestational days (GD) 18.5, 19.5, 20.5, 21.5: external control (EC), exposed to olive oil (OO), exposed to 100 mg nitrofen, by gavage, without CDH (N-), and exposed to nitrofen with CDH (CDH) on GD 9.5 (term=22 days). The morphological variables studied were: body weight (BW), total lung weight (TLW), left lung weight, TLW/BW ratio, total lung volume, and left lung volume. The histometric variables studied were: left lung parenchymal area density and left lung parenchymal volume. VEGFR1 and VEGFR2 expression were determined by Western blotting. The data were analyzed using analysis of variance with the Tukey-Kramer post hoc test. CDH frequency was 37% (80/216). All the morphological and histometric variables were reduced in the N- and CDH groups compared with the controls, and reductions were more pronounced in the CDH group (P<0.05) and more evident on GD 20.5 and GD 21.5. Similar results were observed for VEGFR1 and VEGFR2 expression. We conclude that N- and CDH fetuses showed primary pulmonary hypoplasia, with a decrease in VEGFR1 and VEGFR2 expression. (AU) | |
| FAPESP's process: | 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen |
| Grantee: | Lourenço Sbragia Neto |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/12587-0 - Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats |
| Grantee: | Frances Lilian Lanhellas Gonçalves |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |