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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lateral Habenula and the Rostromedial Tegmental Nucleus Innervate Neurochemically Distinct Subdivisions of the Dorsal Raphe Nucleus in the Rat

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Author(s):
Sego, Chemutai [1, 2] ; Goncalves, Luciano [1, 3] ; Lima, Leandro [1] ; Furigo, Isadora C. [1] ; Donato, Jr., Jose [1] ; Metzger, Martin [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 Sao Paulo - Brazil
[3] Univ Fed Triangulo Mineiro, Dept Human Anat, BR-38025180 Uberaba - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF COMPARATIVE NEUROLOGY; v. 522, n. 7, p. 1454-1484, MAY 1 2014.
Web of Science Citations: 53
Abstract

The lateral habenula (LHb) is an epithalamic structure differentiated in a medial (LHbM) and a lateral division (LHbL). Together with the rostromedial tegmental nucleus (RMTg), the LHb has been implicated in the processing of aversive stimuli and inhibitory control of monoamine nuclei. The inhibitory LHb influence on midbrain dopamine neurons has been shown to be mainly mediated by the RMTg, a mostly GABAergic nucleus that receives a dominant input from the LHbL. Interestingly, the RMTg also projects to the dorsal raphe nucleus (DR), which also receives direct LHb projections. To compare the organization and transmitter phenotype of LHb projections to the DR, direct and indirect via the RMTg, we first placed injections of the anterograde tracer Phaseolus vulgaris leucoagglutinin into the LHb or the RMTg. We then confirmed our findings by retrograde tracing and investigated a possible GABAergic phenotype of DR-projecting RMTg neurons by combining retrograde tracing with in situ hybridization for GAD67. We found only moderate direct LHb projections to the DR, which mainly emerged from the LHbM and were predominantly directed to the serotonin-rich caudal DR. In contrast, RMTg projections to the DR were more robust, emerged from RMTg neurons enriched in GAD67 mRNA, and were focally directed to a distinctive DR subdivision immunohistochemically characterized as poor in serotonin and enriched in presumptive glutamatergic neurons. Thus, besides its well-acknowledged role as a GABAergic control center for the ventral tegmental area (VTA)-nigra complex, our findings indicate that the RMTg is also a major GABAergic relay between the LHb and the DR. J. Comp. Neurol. 522:1454-1484, 2014. (c) 2013 Wiley Periodicals, Inc. (AU)

FAPESP's process: 11/03292-7 - Circuitry and neurochemical signature of the projections among the lateral habenula, the rostromedial tegmental nucleus and the dorsal raphe nucleus
Grantee:Chemutai Sego
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 10/18086-0 - Molecular basis of leptin resistance
Grantee:Jose Donato Junior
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/02388-3 - Topography and transmitter phenotype of the projections between the lateral habenula, the rostromedial tegmental nucleus and the dorsal raphe nucleus in the rat
Grantee:Martin Andreas Metzger
Support Opportunities: Regular Research Grants