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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased Age Is Associated With Epigenetic and Structural Changes in Chromatin From Neuronal Nuclei

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Author(s):
Rodrigues, Henrique F. [1] ; Souza, Tafarel A. [1] ; Ghiraldini, Flavia G. [2] ; Mello, Maria Luiza S. [2] ; Moraes, Alberto S. [1]
Total Authors: 5
Affiliation:
[1] Univ Fed Uberlandia, Inst Biomed Sci, Cytol Histol & Embryol Sect, BR-38400902 Uberlandia, MG - Brazil
[2] Univ Campinas Unicamp, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Cellular Biochemistry; v. 115, n. 4, p. 659-665, APR 2014.
Web of Science Citations: 7
Abstract

Chromatin organization has been considered to play a major role on aging, by regulating DNA accessibility to transcription and repair machinery. Such organization can be modulated by epigenetic events, such as DNA methylation and histone post-translational modifications. Since changes on gene expression profiles have been described in aged neurons, our aim was to study the age-dependent relationship between structural and epigenetic alterations on chromatin of cortical neurons from mice. For this purpose, isolated neuronal nuclei from mice of two ages were studied by image analysis after cytochemistry, or assessed for chromatin accessibility by enzymatic digestion. Additionally, two epigenetic marks, for open and for densely packed chromatin fibers were quantified. Results indicate epigenetically driven alterations on chromatin organization of cortical neurons with advancing age, whose fibers seem to undergo redistribution and unpackaging. Since increased transcriptional activity is not characteristic of aged neurons, these loosened chromatin fibers may be associated with impaired genome stability, as well as with increased accessibility of repair machinery to a life span damaged DNA. J. Cell. Biochem. 115: 659-665, 2014. (c) 2013 Wiley Periodicals, Inc. (AU)

FAPESP's process: 10/50015-6 - Chromatin structure and organization with aging and diabetes compared to induced changes in epigenetic markers
Grantee:Maria Luiza Silveira Mello
Support Opportunities: Research Projects - Thematic Grants