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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Decreased expression of proteins involved in energy metabolism in the hippocampal granular layer of rats submitted to the pilocarpine epilepsy model

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Author(s):
Araujo, Bruno [1, 2] ; Torres, Laila [1, 2, 3] ; Stein, Mariana [4] ; Cabral, Francisco Romero [3, 5] ; Herai, Roberto [2] ; Okamoto, Oswaldo [6] ; Cavalheiro, Esper [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Neurol Expt Escola Paulista Med, BR-04023900 Sao Paulo - Brazil
[2] Univ Calif San Diego, Dept Pediat, Dept Cellular & Mol Med, Childrens Hosp San Diego, Sch Med, Stem Cell Progra, La Jolla, CA 92093 - USA
[3] Hosp Israelita Albert Einstein, Inst Cerebra, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Escola Paulista Med, Lab Biol Mol Parasitas, BR-04023900 Sao Paulo - Brazil
[5] Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo - Brazil
[6] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-09500900 Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Neuroscience Letters; v. 561, p. 46-51, FEB 21 2014.
Web of Science Citations: 5
Abstract

Long-term structural and functional changes in the hippocampus have been identified as the primary physiopathological basis for temporal lobe epilepsy. These changes include reactive gliosis and granule cell axonal sprouting within the dentate gyrus. The intimate mechanisms of these changes are beginning to be revealed. Here, we show the possibility of using laser capture microdissection (LCM) to isolate the dentate granular cell layer of Wistar rats submitted to the pilocarpine model of epilepsy. Using two-dimensional gel electrophoresis (2-D PAGE) and mass spectrometry for laser-captured cells, we identified molecular events that could be altered as part of the epileptic pathogenic process. According to our results, eight proteins related to energy metabolism were differentially expressed between both the control and pilocarpine-treated animals. These results provide, for the first time, new molecular insights into the altered protein profile of the epileptogenic dentate gyrus and can contribute to a better understanding of the phenomena involved in the genesis and maintenance of the epileptic state. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

FAPESP's process: 09/05764-3 - Proteome study of granular gyrus dentate cells from hippocampus of Wistar rats submitted to experimental model of mesial temporal lobe epilepsy
Grantee:Bruno Henrique Silva Araujo Torres
Support type: Scholarships in Brazil - Doctorate