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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Endothelial Activation by Platelets from Sickle Cell Anemia Patients

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Proenca-Ferreira, Renata [1] ; Brugnerotto, Ana Flavia [1] ; Garrido, Vanessa Tonin [1] ; Dominical, Venina Marcela [1] ; Vital, Daiana Morelli [1] ; Reis Ribeiro, Marilene de Fatima [2] ; dos Santos, Melissa Ercolin [2] ; Traina, Fabiola [1, 3] ; Olalla-Saad, Sara T. [1] ; Costa, Fernando Ferreira [1] ; Conran, Nicola [1]
Total Authors: 11
Affiliation:
[1] Univ Campinas UNICAMP, Sch Med, INCT Sangue, Haematol & Haemotherapy Ctr, Campinas, SP - Brazil
[2] Childrens Ctr Hematol Invest, Campinas, SP - Brazil
[3] Univ Sao Paulo, Riberao Preto Med Sch, Dept Internal Med, BR-14049 Ribeirao Preto - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 9, n. 2 FEB 13 2014.
Web of Science Citations: 18
Abstract

Sickle cell anemia (SCA) is associated with a hypercoagulable state. Increased platelet activation is reported in SCA and SCA platelets may present augmented adhesion to the vascular endothelium, potentially contributing to the vaso-occlusive process. We sought to observe the effects of platelets (PLTs) from healthy control (CON) individuals and SCA individuals on endothelial activation, in vitro. Human umbilical vein endothelial cells (HUVEC) were cultured, in the presence, or not, of washed PLTs from CON or steady-state SCA individuals. Supernatants were reserved for cytokine quantification, and endothelial adhesion molecules (EAM) were analyzed by flow cytometry; gene expressions of ICAM1 and genes of the NF-kappa B pathway were analyzed by qPCR. SCA PLTs were found to be more inflammatory, displaying increased adhesive properties, an increased production of IL-1 beta and a tendency towards elevated expressions of P-selectin and activated alpha(IIbb)beta(3). Following culture in the presence of SCA PLTs, HUVEC presented significant augmentations in the expressions of the EAM, ICAM-1 and E-selectin, as well as increased IL-8 production and increased ICAM1 and NFKB1 (encodes p50 subunit of NF-kappa B) gene expressions. Interestingly, transwell inserts abolished the effects of SCA PLTs on EAM expression. Furthermore, an inhibitor of the NF-kappa B pathway, BAY 11-7082, also prevented the induction of EAM expression on the HUVEC surface by SCA PLTs. In conclusion, we find further evidence to indicate that platelets circulate in an activated state in sickle cell disease and are capable of stimulating endothelial cell activation. This effect appears to be mediated by direct contact, or even adhesion, between the platelets and endothelial cells and via NF kappa B-dependent signaling. As such, activated platelets in SCD may contribute to endothelial activation and, therefore, to the vaso-occlusive process. Results provide further evidence to support the use of anti-platelet approaches in association with other therapies for SCD. (AU)

FAPESP's process: 08/57441-0 - Clinical, cellular and molecular alterations in hemoglobinopathies and other hereditary hemolytic anemias
Grantee:Fernando Ferreira Costa
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/54279-0 - Interactions between platelets from sickle cell anemia patients and human umbilical vein endothelial cells (HUVEC): in vitro evaluation of platelets adhesion and their effects on the endothelial activation
Grantee:Renata Proenca Ferreira
Support type: Scholarships in Brazil - Doctorate