Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of P-MAPA immunomodulator on Toll-like receptor 2, ROS, nitric oxide, MAPKp38 and IKK in PBMC and macrophages from dogs with visceral leishmaniasis

Full text
Author(s):
Melo, L. M. [1] ; Perosso, J. [1] ; Almeida, B. F. M. [1] ; Silva, K. L. O. [1] ; Somenzari, M. A. [2] ; de Lima, V. M. F. [2]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Fac Med Vet, Posgrad Ciencia Anim, Aracatuba, SP - Brazil
[2] Univ Estadual Paulista, Fac Med Vet, Dept Clin Cirugia & Reprod Anim, Aracatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: International Immunopharmacology; v. 18, n. 2, p. 373-378, FEB 2014.
Web of Science Citations: 13
Abstract

Leishmania (L.) chagasi is the etiologic agent of visceral leishmaniasis (VL) that can be transmitted to humans and dogs. VL in Brazil represents a serious public health problem; therefore, it is important to study new alternatives to treat infected dogs. In dogs, the therapeutic arsenal against canine VL is limited. The immunomodulator protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride (P-MAPA) improves immunocompetence when the immune system is impaired, but its dependence on Toll-like receptors (TLRs) and the mechanisms involved in immune response remain unclear. The in vitro action of P-MAPA on the expression of TLR2 and TLR4, reactive oxygen species (ROS), nitric oxide (NO) and p38 mitogen-activated protein kinase (p38 MAPK) and IKK phosphorylation was studied in mononuclear cells from peripheral blood and macrophages from healthy and Leishmania-infected dogs. The PBMC or macrophages were isolated and cultured with different concentrations of P-MAPA (20,100 and 200 mu g/ml) in a humid environment at 37 degrees C with 5% CO2. Observation revealed that Leishmania-infected dogs showed a decrease in TLR2 in macrophages compared with healthy dogs and in induction with P-MAPA. ROS were increased in PBMCs from Leishmania spp.-infected dogs compared with healthy dogs and P-MAPA improved ROS production. NO production was increased in culture supernatant from macrophages stimulated by P-MAPA in both healthy and Leishmania spp. infected dogs. Treatment of macrophages from healthy dogs with immunomodulatory P-MAPA induced p38 MAPK and IKK phosphorylation, suggesting signal transduction by this pathway. These findings suggest that P-MAPA has potential as a therapeutic drug in the treatment of canine visceral leishmaniasis. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/50426-9 - Use evaluation of protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride immunomodulator in the treatment of dogs with symptoms of visceral leishmaniasis
Grantee:Valéria Marçal Felix de Lima
Support type: Regular Research Grants
FAPESP's process: 10/13166-6 - TL2 and TL4 evaluation, ROS, and nitric oxide in peripheral blood mononuclear cells from Leishmania infected dogs and controls after stimluation with P-MAPA immunomodulator
Grantee:Larissa Martins Melo
Support type: Scholarships in Brazil - Master