| Full text | |
| Author(s): |
Rosa-Ribeiro, Rafaela
[1]
;
Barbosa, Guilherme Oliveira
[1]
;
Kuehne, Fabiana
[1]
;
Carvalho, Hernandes F.
[1]
Total Authors: 4
|
| Affiliation: | [1] State Univ Campinas UNICAMP, Inst Biol, Dept Cell Biol, BR-13083863 Campinas, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Histochemistry and Cell Biology; v. 141, n. 2, p. 213-220, FEB 2014. |
| Web of Science Citations: | 8 |
| Abstract | |
The mechanism underlying castration-induced prostate regression, which is a classical physiological concept translated into the therapeutic treatment of advanced prostate cancer, involves epithelial cell apoptosis. In searching for events and mechanisms contributing to prostate regression in response to androgen modulation, we have frequently observed the collective deletion of epithelial cells. This work was undertaken to characterize this phenomenon hereafter named desquamation and to verify its presence after 17 beta-estradiol (E2) administration. Electron microscopy revealed that the desquamating cells had preserved cell-cell junctions and collapsed nuclear contents. The TUNEL reaction was negative for these cells, which were also negative for cleaved caspases-8, -9, -3 and nuclear apoptosis-inducing factor. Detailed analyses revealed that the condensed chromatin was first affected detaching from the nuclear lamina, which was observable after lamin A immunohistochemistry, suggesting the lack of lamin A degradation. A search in animals treated with supraphysiological E2 employed as an alternative anti-androgen treatment revealed no desquamation. The combined treatment (Cas + E2 group) caused changes particular to each treatment, including desquamation. In conclusion, desquamation appeared as a novel phenomenon contributing to collective prostate epithelial cell deletion, distinct from the classical castration-induced apoptosis and particular to the androgen deprivation resulting from surgical castration, and should be considered as part of the mechanisms promoting organ regression. (AU) | |
| FAPESP's process: | 08/07990-8 - Identification of the signaling pathways involved with epithelial cell death in the rat ventral prostate in response to castration and estrogen administration |
| Grantee: | Rafaela da Rosa Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 09/16150-6 - Androgen regulation, sinalization and cellular interactions in prostate development, physiology and regression |
| Grantee: | Hernandes Faustino de Carvalho |
| Support Opportunities: | Research Projects - Thematic Grants |