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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Developmental rates of immatures of three Chrysomya species (Diptera: Calliphoridae) under the effect of methylphenidate hydrochloride, phenobarbital, and methylphenidate hydrochloride associated with phenobarbital

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Author(s):
Rezende, Fabio [1] ; Alonso, Marcela A. [1] ; Souza, Carina M. [1] ; Thyssen, Patricia J. [2] ; Linhares, Aricio X. [1]
Total Authors: 5
Affiliation:
[1] Univ Campinas UNICAMP, Dept Anim Biol, BR-13083970 Campinas, SP - Brazil
[2] Fed Univ Pelotas UFPel, Dept Microbiol & Biol, BR-96010900 Pelotas, RS - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Parasitology Research; v. 113, n. 5, p. 1897-1907, MAY 2014.
Web of Science Citations: 6
Abstract

Entomotoxicology is focused on obtaining data on necrophagous entomofauna, for criminal investigations purposes. This study aimed to evaluate the effect of different concentrations of methylphenidate hydrochloride, phenobarbital, and their association on the developmental rate, larval and pupal survivorship, and the interval of emergence of adults of Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius), and Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae). Considering the therapeutic dose (TD) of methylphenidate hydrochloride (0.29 mg/Kg), the concentrations tested were 10x TD, 50x TD, and 100x TD. For phenobarbital, the concentrations used were 1x TD (=150 mg/Kg), 3.3x TD, and 6.7x TD. For the association of the drugs, the combinations used were 10x TD-methylphenidate hydrochloride plus 1x TD-phenobarbital, 50x TD-methylphenidate hydrochloride plus 3.3x TD-phenobarbital, and 100x TD-methylphenidate hydrochloride plus 6.7x TD-phenobarbital. The control group, without addition of drug, was maintained under the same conditions of temperature (25 +/- 1 A degrees C), humidity (70 A +/- 10 %), and photoperiod (12 h). Specimens of each group were weighed every 12 h until pupariation. The developmental rate of the three Chrysomya species immatures was monitored. For C. albiceps the developmental time was delayed in 24 h for methylphenidate hydrochloride group and in 12 h for the phenobarbital and the drugs association groups. The effect was observed only at specific ages for C. megacephala, without altering the developmental time. For C. putoria, the developmental time was delayed in 12 h for methylphenidate hydrochloride group and in 24 h for the phenobarbital and the drugs association groups. The emergence interval was similar among all experimental groups, but larval and pupal viabilities were affected in different ways. (AU)

FAPESP's process: 11/02846-9 - Standarzation of immunohistochemistry method for detection of drugs in larvae tissues of dipterans of forensic importance
Grantee:Marcela Aquiyama Alonso
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/03865-4 - Evaluation of the developmental rate of three species of Chrysomya immatures (Diptera: Calliphoridae) under the influence of methylphenidate and methylphenidate associated to phenobarbital
Grantee:Fabio Rezende
Support Opportunities: Scholarships in Brazil - Master