| Full text | |
| Author(s): |
de Moraes, Marcela Cristina
[1]
;
Temporini, Caterina
[2]
;
Calleri, Enrica
[2]
;
Bruni, Giovanna
[3]
;
Ducati, Rodrigo Gay
[4]
;
Santos, Diogenes Santiago
[4]
;
Cardoso, Carmen Lucia
[5]
;
Cass, Quezia Bezerra
[1]
;
Massolini, Gabriella
[2]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Paulo - Brazil
[2] Univ Pavia, Dept Drug Sci, I-27100 Pavia - Italy
[3] Univ Pavia, Dept Chem, I-27100 Pavia - Italy
[4] Pontificia Univ Catolica Rio Grande do Sul PUCRS, Ctr Pesquisas Biol Mol & Func, Inst Nacl Ciencia & Tecnol TB, Porto Alegre, RS - Brazil
[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Journal of Chromatography A; v. 1338, p. 77-84, APR 18 2014. |
| Web of Science Citations: | 9 |
| Abstract | |
The aim of this work was to optimize the preparation of a capillary human purine nucleoside phosphorylase (HsPNP) immobilized enzyme reactor (IMER) for characterization and affinity screening studies of new inhibitors by frontal affinity chromatography coupled to mass spectrometry (FAC-MS). For this purpose two monolithic supports, a Chromolith Speed Rod (0.1 mm I.D. x 5 cm) and a methacrylate-based monolithic epoxy polymeric capillary column (0.25 mm I.D. x 5 cm) with epoxy reactive groups were considered and compared to an IMER previously developed using an open fused silica capillary. Each HsPNP-IMER was characterized in terms of catalytic activity using Inosine as standard substrate. Furthermore, they were also explored for affinity ranking experiments. K-d determination was carried out with the based fused silica HsPNP-IMER and the results are herein discussed. (C) 2014 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 08/04051-0 - Development and application of capillary bioreactors for the screening inhibitors of Purine Nucleoside Phosphorylase |
| Grantee: | Marcela Cristina de Moraes |
| Support Opportunities: | Scholarships in Brazil - Doctorate |