Glutamate requires NMDA receptors to modulate alpha2 adrenoceptor in medulla oblongata cultured cells of newborn rats

Author(s)

da Silva, Sergio Marinho ^[1] ; Carrettiero, Daniel C. ^[2] ; Chadi, Debora R. F. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Physiol, Inst Biosci, BR-05508090 Sao Paulo - Brazil

^[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, Santo Andre - Brazil

• Document type: Journal article
• Source: Neuroscience Letters; v. 564, p. 83-88, APR 3 2014.
• Web of Science Citations: 0

Abstract

alpha 2 Adrenoceptors (alpha 2-ARs) are important in regulating the central control of blood pressure in medulla oblongata. However, it is unclear how this receptor is modulated by different receptors, especially the glutamatergic. In the present study, we studied the influence of ionotropic glutamatergic receptors over the alpha 2-ARs in cultured cells of the medulla oblongata of newborn rats. For this purpose, the protein level of the alpha 2-ARs was assessed after administration to the cultured cells of glutamate (glu), the agonists NMDA and kainate (KA), the NMDA receptor antagonist MK801 and the MA receptor antagonist DNQX. Results indicate that the alpha 2-AR protein levels were increased after the treatments with glu and NMDA, and the addition of MK801 to this treatment thwarted this increase. Notwithstanding the fact that MA did not alter the receptor protein level, the combined treatment of DNQX with glu prevented the alpha 2-AR protein modulation. In conclusion, the present study suggests that ionotropic glutamatergic receptors could be related to the alpha 2-AR protein regulation in the medulla oblongata. (C) 2014 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 10/19849-8 - Study of the mechanisms involved in neurotransmitter release: analysis through A2a adenosine receptor activation
• Grantee: Debora Rejane Fior Chadi
• Support Opportunities: Regular Research Grants