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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

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Author(s):
Damous, Luciana Lamarao [1] ; Nakamuta, Juliana Sanajotti [2] ; Soares-, Jr., Jose Maria [1] ; Rosa Maciel, Gustavo Arantes [1] ; Simoes, Ricardo dos Santos [1] ; de Souza Montero, Edna Frasson [3] ; Krieger, Jose Eduardo [2] ; Baracat, Edmund Chada [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Med, Dept Obstet & Gynecol, Lab Struct & Mol Gynecol LIM 58, Gynecol Div, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Heart Inst Incor, Lab Genet & Mol Cardiol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med, Dept Surg, Lab Surg Physiopathol LIM 62, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF OVARIAN RESEARCH; v. 7, MAR 20 2014.
Web of Science Citations: 4
Abstract

Background: Cryopreservation of the ovarian tissue has shown promising results. However, there remain controversial issues such as the short half-life of grafts. In this aspect, there are some evidences that preconditioning the ovarian tissue before transplantation is beneficial. Objective: To determine the effect of hypoxic preconditioning in vitro on ovarian tissue prior to transplantation. Methods: Eighteen female adult Wistar rats, were sorted into three experimental groups. Ovaries were maintained in DMEM low glucose serum free at 37 degrees C with 5% CO2, at atmospheric oxigen concentration (normoxia) or 1% O-2 (hypoxia) for 16 hours. Oxigen concentration was determined by injection of nitrogen in the incubator. Animals submitted to ovarian transplantation immediately after oophorectomy were the Control Group (C). After this, the ovaries were implanted in the retroperitoneum with nonabsorbable suture and animals evaluated for thirty days after transplantation. Beginning on postoperative (PO) day 11, a daily collection of vaginal smear was carried out. Analyses comprised morphological, morphometric (counting ovarian follicles and corpora lutea) and immunohistochemistry for cleaved caspase-3 (apoptosis). Results: In normoxia and control groups all animals recovered their estrous cycles, while in the hypoxia group, two animals did not ovulate but, among those which did, resumption took longer than in the other groups (p < 0.05). The number of ovarian follicles and corpora lutea decreased significantly in the hypoxia group when compared to the other two groups (p < 0.001) and apoptosis was increased in the few ovarian follicles which remained viable (p < 0.001). Conclusion: The hypoxic preconditioning in vitro was not beneficial to the graft and worsened their viability, compromising its functionality or delaying the return of this. (AU)

FAPESP's process: 12/09469-9 - Cellular and gene therapy in experimental fresh and cryopreserved ovary transplant
Grantee:Edmund Chada Baracat
Support Opportunities: Regular Research Grants
FAPESP's process: 10/17897-5 - Cellular and gene therapy in experimental fresh and cryopreserved ovary transplant
Grantee:Luciana Lamarão Damous
Support Opportunities: Scholarships in Brazil - Post-Doctoral