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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immobilization and therapeutic passive stretching generate thickening and increase the expression of laminin and dystrophin in skeletal muscle

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Author(s):
L.O. Cação-Benedini [1] ; P.G. Ribeiro [2] ; C.M. Prado [3] ; D.L. Chesca [3] ; A.C. Mattiello-Sverzut [2]
Total Authors: 5
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Medicina e Reabilitação do Aparelho Locomotor - Brasil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biomecan Med & Reabilitacao Aparelho Locomot, BR-14049 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 47, n. 6, p. 483-491, 2014-05-09.
Abstract

Extracellular matrix and costamere proteins transmit the concentric, isometric, and eccentric forces produced by active muscle contraction. The expression of these proteins after application of passive tension stimuli to muscle remains unknown. This study investigated the expression of laminin and dystrophin in the soleus muscle of rats immobilized with the right ankle in plantar flexion for 10 days and subsequent remobilization, either by isolated free movement in a cage or associated with passive stretching for up to 10 days. The intensity of the macrophage response was also evaluated. One hundred and twenty-eight female Wistar rats were divided into 8 groups: free for 10 days; immobilized for 10 days; immobilized/free for 1, 3, or 10 days; or immobilized/stretched/free for 1, 3, or 10 days. After the experimental procedures, muscle tissue was processed for immunofluorescence (dystrophin/laminin/CD68) and Western blot analysis (dystrophin/laminin). Immobilization increased the expression of dystrophin and laminin but did not alter the number of macrophages in the muscle. In the stretched muscle groups, there was an increase in dystrophin and the number of macrophages after 3 days compared with the other groups; dystrophin showed a discontinuous labeling pattern, and laminin was found in the intracellular space. The amount of laminin was increased in the muscles treated by immobilization followed by free movement for 10 days. In the initial stages of postimmobilization (1 and 3 days), an exacerbated macrophage response and an increase of dystrophin suggested that the therapeutic stretching technique induced additional stress in the muscle fibers and costameres. (AU)

FAPESP's process: 09/53342-0 - Study of costameric proteins in soieus muscle longitudinal sections of female rats immobilized and rehabilitated by intermittent passive manual stretching
Grantee:Ana Cláudia Mattiello-Sverzut
Support Opportunities: Regular Research Grants