| Full text | |
| Author(s): |
Ribeiro, Livia C. A.
[1]
;
Massimino, Livia C.
[1]
;
Durante, Araceli C.
[2]
;
Tansini, Aline
[1]
;
Urbaczek, Ana C.
[1]
;
Selistre-de-Araujo, Heloisa S.
[2]
;
Carlos, Iracilda Z.
[1]
Total Authors: 7
|
| Affiliation: | [1] Sao Paulo State Univ, Clin Immunol Lab, Dept Clin Anal, Coll Pharmaceut Sci, Sao Paulo - Brazil
[2] Univ Fed Sao Carlos, Dept Physiol Sci, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | CELL ADHESION & MIGRATION; v. 8, n. 1, p. 60-65, JAN 1 2014. |
| Web of Science Citations: | 4 |
| Abstract | |
Integrin alpha v beta 3 is most likely the foremost modulator of angiogenesis among all known integrins. Recombinant disintegrin DisBa-01, originally obtained from snake venom glands, binds to alpha v beta 3, thereby significantly inhibiting adhesion and generating in vivo anti-metastatic ability. However, its function in mediator production is not clear. Here, we observed that the mediators VEGF-A, IL-8, and TGF-beta are not produced by human umbilical vein endothelial cells (HUVEC cell line) or monocyte/macrophage cells (SC cell line) when cells adhered to vitronectin. However, when exposed to DisBa-01, HUVECs produced higher levels of TGF-beta, and SC cells produced higher levels of VEGF-A. Nonetheless, HUVECs also showed an enhancement of apoptosis after losing adherence when exposed to disintegrin, which is a characteristic of anoikis. We propose that disintegrin DisBa-01 could be used to modulate integrin alpha v beta 3 functions. (AU) | |
| FAPESP's process: | 10/05428-0 - Recombinant desintegrin DisBa-01 effect in liposome and non-liposome form on macrophages and neoplasic cells: evaluation of cytokine production and pro-angiogenic factors |
| Grantee: | Iracilda Zeppone Carlos |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/07798-2 - Effect of DisBa-01 recombinant disintegrin on macrophages and tumour cells: apoptosis evaluation |
| Grantee: | Lívia Contini Massimino |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 10/01568-2 - Recombinant desintegrin DisBa-01 effect in liposome and non-liposome form on macrophages and neoplasic cells: evaluation of cytokine production and pro-angiogenic factors |
| Grantee: | Lívia Carolina de Abreu Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |