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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recombinant disintegrin targets alpha(v) beta(3) integrin and leads to mediator production

Full text
Author(s):
Ribeiro, Livia C. A. [1] ; Massimino, Livia C. [1] ; Durante, Araceli C. [2] ; Tansini, Aline [1] ; Urbaczek, Ana C. [1] ; Selistre-de-Araujo, Heloisa S. [2] ; Carlos, Iracilda Z. [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Clin Immunol Lab, Dept Clin Anal, Coll Pharmaceut Sci, Sao Paulo - Brazil
[2] Univ Fed Sao Carlos, Dept Physiol Sci, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CELL ADHESION & MIGRATION; v. 8, n. 1, p. 60-65, JAN 1 2014.
Web of Science Citations: 4
Abstract

Integrin alpha v beta 3 is most likely the foremost modulator of angiogenesis among all known integrins. Recombinant disintegrin DisBa-01, originally obtained from snake venom glands, binds to alpha v beta 3, thereby significantly inhibiting adhesion and generating in vivo anti-metastatic ability. However, its function in mediator production is not clear. Here, we observed that the mediators VEGF-A, IL-8, and TGF-beta are not produced by human umbilical vein endothelial cells (HUVEC cell line) or monocyte/macrophage cells (SC cell line) when cells adhered to vitronectin. However, when exposed to DisBa-01, HUVECs produced higher levels of TGF-beta, and SC cells produced higher levels of VEGF-A. Nonetheless, HUVECs also showed an enhancement of apoptosis after losing adherence when exposed to disintegrin, which is a characteristic of anoikis. We propose that disintegrin DisBa-01 could be used to modulate integrin alpha v beta 3 functions. (AU)

FAPESP's process: 10/05428-0 - Recombinant desintegrin DisBa-01 effect in liposome and non-liposome form on macrophages and neoplasic cells: evaluation of cytokine production and pro-angiogenic factors
Grantee:Iracilda Zeppone Carlos
Support Opportunities: Regular Research Grants
FAPESP's process: 11/07798-2 - Effect of DisBa-01 recombinant disintegrin on macrophages and tumour cells: apoptosis evaluation
Grantee:Lívia Contini Massimino
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/01568-2 - Recombinant desintegrin DisBa-01 effect in liposome and non-liposome form on macrophages and neoplasic cells: evaluation of cytokine production and pro-angiogenic factors
Grantee:Lívia Carolina de Abreu Ribeiro
Support Opportunities: Scholarships in Brazil - Doctorate