| Full text | |
| Author(s): |
Katia Cristina Dantas
[1]
;
Roseli S. Freitas
[2]
;
Adriana Pardini Vicentini Moreira
[3]
;
Marcos Vinicius da Silva
[4]
;
Gil Benard
[5]
;
Cidia Vasconcellos
;
Paulo Ricardo Criado
[7]
Total Authors: 7
|
| Affiliation: | [1] University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine - Brasil
[2] University of São Paulo. Faculty of Medicine. Hospital das Clínicas - Brasil
[3] State Health Secretariat (IAL). Doctor of Microbiology and Immunology, degree awarded by the Federal University of São Paulo (UNIFESP) - Adolfo Lutz Institute - Brasil
[4] Doctor, degree awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) – Infectologist of the Emílio Ribas Infectology Institute - Brasil
[5] University of São Paulo. Faculty of Medicine. Post-doctorate - Professor of the Dermatology Department - Brasil
[7] University of São Paulo. Faculty of Medicine. Hospital das Clínicas - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | ANAIS BRASILEIROS DE DERMATOLOGIA; v. 88, n. 1, p. 141-143, 2013-02-00. |
| Abstract | |
The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis. (AU) | |