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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients*

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Author(s):
Katia Cristina Dantas [1] ; Roseli S. Freitas [2] ; Adriana Pardini Vicentini Moreira [3] ; Marcos Vinicius da Silva [4] ; Gil Benard [5] ; Cidia Vasconcellos ; Paulo Ricardo Criado [7]
Total Authors: 7
Affiliation:
[1] University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine - Brasil
[2] University of São Paulo. Faculty of Medicine. Hospital das Clínicas - Brasil
[3] State Health Secretariat (IAL). Doctor of Microbiology and Immunology, degree awarded by the Federal University of São Paulo (UNIFESP) - Adolfo Lutz Institute - Brasil
[4] Doctor, degree awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) – Infectologist of the Emílio Ribas Infectology Institute - Brasil
[5] University of São Paulo. Faculty of Medicine. Post-doctorate - Professor of the Dermatology Department - Brasil
[7] University of São Paulo. Faculty of Medicine. Hospital das Clínicas - Brasil
Total Affiliations: 7
Document type: Journal article
Source: ANAIS BRASILEIROS DE DERMATOLOGIA; v. 88, n. 1, p. 141-143, 2013-02-00.
Abstract

The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis. (AU)