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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of fluoxetine and norfluoxetine in human plasma by HPLC-UV using a high purity C18 silica-based SPE sorbent

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Author(s):
Domingues Nazario, Carlos Eduardo [1] ; de Lima Gomes, Paulo Clairmont Feitosa [1] ; Mauro Lancas, Fernando [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Chem Sao Carlos, Lab Chromatog, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ANALYTICAL METHODS; v. 6, n. 12, p. 4181-4187, 2014.
Web of Science Citations: 8
Abstract

This paper reports on the development and validation of a simple and sensitive method that uses solid phase extraction (SPE) and liquid chromatography with ultraviolet detection to analyze fluoxetine (FLX) and norfluoxetine (NFLX) in human plasma samples. A lab-made C18 SPE phase was synthesized by using a sol-gel process employing a low-cost silica precursor. This sorbent was fully characterized by nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM) to check the particles' shape, size and C18 functionalization. The lab-made C18 silica was used in the sample preparation step of human plasma by the SPE-HPLC-UV method. The method was validated in the 15 to 500 ng mL(-1) range for both FLX and NFLX using a matrix matched curve. Detection limits of 4.3 and 4.2 ng mL(-1) were obtained for FLX and NFLX, respectively. The repeatability and intermediary precision achieved varied from 7.6 to 15.0% and the accuracy ranged from -14.9 to 9.1%. The synthesized C18 sorbent was compared to commercial C18 sorbents. The average recoveries were similar (85-105%), however the lab-made C18 silica showed fewer interfering peaks in the chromatogram. After development and validation, the method using the lab-made C18 SPE was applied to plasma samples of patients under FLX treatment (n = 6). The concentrations of FLX and NFLX found in the samples varied from 46.8-215.5 and 48.0-189.9 ng mL(-1), respectively. (AU)

FAPESP's process: 05/59360-0 - Miniaturization of analytical instrumentation for separation techniques: sample preparation and printing of result
Grantee:Fernando Mauro Lanças
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 07/03844-4 - Simultaneous analysis of multi-residue antibiotics in water and sediments by headspace bar sorptive extraction with in situ derivatization coupled to GC / MS
Grantee:Paulo Clairmont Feitosa de Lima Gomes
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)