Standardization of a protocol to extract and analyze chlorophyll a and carotenoids in Gracilaria tenuistipitata Var. Liui. Zhang and Xia (Rhodophyta)

Author(s)

Torres, Priscila Bezerra ^[1] ; Chow, Fungyi ^[1] ; Furlan, Claudia Maria ^[2] ; Mandelli, Fernanda ^[2] ; Mercadante, Adriana ^[2] ; Alves Cursino dos Santos, Deborah Yara ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo - Brazil

^[2] Univ Estadual Campinas Unicamp, Inst Biol, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Oceanography; v. 62, n. 1, p. 57-63, JAN-MAR 2014.
• Web of Science Citations: 10

Abstract

Chlorophyll a and carotenoids are important pigments in photosynthesis. Several studies have been published describing extraction and analysis protocols of these pigments, mainly in vascular plant species. This study standardizes an extraction and analysis protocol of these substances in Gracilaria tenuistipitata var. liui, a red seaweed. Apical portions grown in vitro were triturated in liquid nitrogen. Extracts were prepared in 1.5 mL solvent and centrifuged. Quantitative and qualitative analyses of pigments were performed by UV/visible light spectrophotometry and high performance liquid chromatography (HPLC) and HPLC coupled to mass spectrometry (HPLC-MS). The parameters assessed were: minimum biomass, best extraction solvent, and number of extraction steps. Methanol was the most efficient solvent, and 50 mg fresh biomass was the amount of sample indicated, submitted to one single extraction step. No significant differences were observed in levels of these pigments by UV-visible light spectrophotometry and HPLC. However, HPLC or HPLC-MS are required to identify the different carotenoids present in this seaweed species. (AU)

• FAPESP's process: 10/02948-3 - Integrative physiology in macroalgae: exploratory study of chemical biodiversity
• Grantee: Fanly Fungyi Chow Ho
• Support Opportunities: Regular Research Grants