| Full text | |
| Author(s): |
Carla Roberta Freschi
[1]
;
Luiz Fernando de Oliveira e Silva Carvalho
[2]
;
Celso José Bruno de Oliveira
[3]
Total Authors: 3
|
| Affiliation: | [1] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Clínica e Cirurgia Veterinária - Brasil
[2] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Clínica e Cirurgia Veterinária - Brasil
[3] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Animal - Brasil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Microbiology; v. 36, n. 4, p. 363-367, 2005-12-00. |
| Abstract | |
The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P<0.05) when combined to Rappaport-Vassiliadis. Considering cost and efficiency parameters, we encourage the use of Müller-Kauffmann tetrathionate broth in combination with boiling-centrifugation DNA-extraction procedure. (AU) | |