| Full text | |
| Author(s): |
João Gabriel Martins Dallo
[1]
;
Bernardo Vergara Reichert
[2]
;
José Benedito Ramos Valladão Júnior
[3]
;
Camila Silva
[4]
;
Bianca Aparecida de Luca
[5]
;
Beatriz de Freitas Azevedo Levy
[6]
;
Gerson Chadi
[7]
Total Authors: 7
|
| Affiliation: | [1] USP. School of Medicine - Brasil
[2] USP. School of Medicine - Brasil
[3] USP. School of Medicine - Brasil
[4] USP. Psychology Institute. Neuroscience and Behavior - Brasil
[5] USP. Psychology Institute. Neuroscience and Behavior - Brasil
[6] USP. Psychology Institute. Neuroscience and Behavior - Brasil
[7] USP. School of Medicine. Department of Neurology - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Acta Cirurgica Brasileira; v. 22, n. 6, p. 485-494, 2007-12-00. |
| Abstract | |
PURPOSE: Reactive astrocytes are implicated in several mechanisms after central or peripheral nervous system lesion, including neuroprotection, neuronal sprouting, neurotransmission and neuropathic pain. Schwann cells (SC), a peripheral glia, also react after nerve lesion favoring wound/repair, fiber outgrowth and neuronal regeneration. We investigated herein whether cell therapy for repair of lesioned sciatic nerve may change the pattern of astroglial activation in the spinal cord ventral or dorsal horn of the rat. METHODS: Injections of a cultured SC suspension or a lesioned spinal cord homogenized extract were made in a reservoir promoted by a contiguous double crush of the rat sciatic nerve. Local injection of phosphate buffered saline (PBS) served as control. One week later, rats were euthanized and spinal cord astrocytes were labeled by immunohistochemistry and quantified by means of quantitative image analysis. RESULTS: In the ipsilateral ventral horn, slight astroglial activations were seen after PBS or SC injections, however, a substantial activation was achieved after cord extract injection in the sciatic nerve reservoir. Moreover, SC suspension and cord extract injections were able to promote astroglial reaction in the spinal cord dorsal horn bilaterally. Conclusion: Spinal cord astrocytes react according to repair processes of axotomized nerve, which may influence the functional outcome. The event should be considered during the neurosurgery strategies. (AU) | |