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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of a laser induced fluorescence detection scheme for capillary electrophoresis

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Author(s):
Marcia R. Santos [1] ; Marina F. M. Tavares [2] ; Joel C. Rubim [3]
Total Authors: 3
Affiliation:
[1] Universidade de São Paulo. Instituto de Química
[2] Universidade de São Paulo. Instituto de Química
[3] Universidade de Brasília. Instituto de Química
Total Affiliations: 3
Document type: Journal article
Source: Química Nova; v. 23, n. 5, p. 585-589, 2000-10-00.
Abstract

A capillary electrophoresis system using laser induced fluorescence detection is described. A Raman system equipped with a microscope has been used to focus the laser beam on the capillary giving a lateral resolution of 1.5 mm. The fluorescence signal of the analyte (ZnPcTS - tetrasulfonated zinc-phthalocyanine) was collected by the microscope objectives and analysed by a monochromator with confocal characteristics equipped with a CCD detector. Electropherograms obtained with this system were compared to those obtained on a commercial instrument, showing that the described system presents a lower detection limit and better resolution. (AU)