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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

LAND-USE SYSTEMS AFFECT ARCHAEAL COMMUNITY STRUCTURE AND FUNCTIONAL DIVERSITY IN WESTERN AMAZON SOILS

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Author(s):
Navarrete, Acacio Aparecido [1] ; Taketani, Rodrigo Gouvea [2] ; Mendes, Lucas William [3] ; Cannavan, Fabiana de Souza [1] ; de Souza Moreira, Fatima Maria [4] ; Tsai, Siu Mui [2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Grad Program Appl Ecol, Luiz Queiroz Coll Agr ESALQ, BR-05508 Sao Paulo - Brazil
[2] Univ Sao Paulo, CENA, Cell & Mol Biol Lab, BR-05508 Sao Paulo - Brazil
[3] Univ Sao Paulo, CENA, Grad Program Sci, BR-05508 Sao Paulo - Brazil
[4] Fed Univ Lavras UFLA, Dept Soil Sci, BR-37200000 Lavras, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Revista Brasileira de Ciência do Solo; v. 35, n. 5, p. 1527-1540, SEP-OCT 2011.
Web of Science Citations: 25
Abstract

The study of the ecology of soil microbial communities at relevant spatial scales is primordial in the wide Amazon region due to the current land use changes. In this study, the diversity of the Archaea domain (community structure) and ammonia-oxidizing Archaea (richness and community composition) were investigated using molecular biology-based techniques in different land-use systems in western Amazonia, Brazil. Soil samples were collected in two periods with high precipitation (March 2008 and January 2009) from Inceptisols under primary tropical rainforest, secondary forest (5-20 year old), agricultural systems of indigenous people and cattle pasture. Denaturing gradient gel electrophoresis of polymerase chain reaction-amplified DNA (PCR-DGGE) using the 16S rRNA gene as a biomarker showed that archaeal community structures in crops and pasture soils are different from those in primary forest soil, which is more similar to the community structure in secondary forest soil. Sequence analysis of excised DGGE bands indicated the presence of crenarchaeal and euryarchaeal organisms. Based on clone library analysis of the gene coding the subunit of the enzyme ammonia monooxygenase (amoA) of Archaea (306 sequences), the Shannon-Wiener function and Simpson's index showed a greater ammonia-oxidizing archaeal diversity in primary forest soils (H' = 2.1486; D = 0.1366), followed by a lower diversity in soils under pasture (H' = 1.9629; D = 0.1715), crops (H' = 1.4613; D = 0.3309) and secondary forest (H' = 0.8633; D = 0.5405). All cloned inserts were similar to the Crenarchaeota amoA gene clones (identity >= 95%) previously found in soils and sediments and distributed primarily in three major phylogenetic clusters. The findings indicate that agricultural systems of indigenous people and cattle pasture affect the archaeal community structure and diversity of ammonia-oxidizing Archaea in western Amazon soils. (AU)

FAPESP's process: 08/58114-3 - Monitoring the microbial diversity and functional activities in response to land-use changes and deforestation under soybean and sugarcane cultivations
Grantee:Tsai Siu Mui
Support type: Research Program on Global Climate Change - Thematic Grants