| Full text | |
| Author(s): |
Mesnard, Laurent
[1]
;
Keller, Alexandre D. C.
[2]
;
Michel, Marie-Laure
[3]
;
Vandermeersch, Sophie
[4]
;
Rafat, Cedric
[5]
;
Letavernier, Emmanuel
[6]
;
Tillet, Yves
[7]
;
Rondeau, Eric
[8]
;
Leite-de-Moraes, Maria C.
[9]
Total Authors: 9
|
| Affiliation: | [1] Hop Tenon. INSERM
[2] Univ Paris 05. Fac Med
[3] Univ Paris 05. Fac Med
[4] Hop Tenon. INSERM
[5] Hop Tenon. INSERM
[6] Hop Tenon. INSERM
[7] INRA. UMR85
[8] Hop Tenon. INSERM
[9] Univ Paris 05. Fac Med
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY; v. 20, n. 6, p. 1282-1292, JUN 2009. |
| Web of Science Citations: | 35 |
| Abstract | |
Invariant natural killer T (iNKT) cells represent a particular subset of T lymphocytes capable of producing several cytokines, which exert regulatory or effector functions, following stimulation of the T cell receptor. In this study, we investigated the influence of iNKT cells on the development of experimental anti-glomerular basement membrane glomerulonephritis (anti-GBM GN). After injection of anti-GBM serum, the number of kidney iNKT cells rapidly increased. iNKT cell-deficient mice (J alpha 18(-/-)) injected with anti-GBM serum demonstrated worse renal function, increased proteinuria, and greater glomerular and tubular injury compared with similarly treated wild-type mice. We did not detect significant differences in Th1/Th2 polarization in renal tissue that might have explained the severity of disease in J alpha 18(-/-) mice. Interestingly, expression of both TGF-beta and TGF-beta-induced (TGFBI) mRNA was higher in wild-type kidneys compared with J alpha 18(-/-) kidneys, suggesting a possible protective role for TGF-beta in anti-GBM GN. Administration of an anti-TGF-beta neutralizing antibody significantly enhanced the severity of disease in wild-type, but not J alpha 18(-/-), mice. In conclusion, in experimental anti-GBM GN, iNKT cells attenuate disease severity and TGF-beta has a renoprotective role. (AU) | |
| FAPESP's process: | 07/07120-0 - The role of invariant natural killer T cells in the development of glomerulonephritis: mechanisms and perspectives |
| Grantee: | Alexandre de Castro Keller |
| Support Opportunities: | Research Grants - Young Investigators Grants |