| Full text | |
| Author(s): |
de Souza Sobrinho, Celestino Prospero
[1]
;
Gragnani, Alfredo
[2]
;
Abranches Oliveira Santos, Ivan Dunshee
[3]
;
Oliveira, Andrea Fernandes
[4]
;
Nunes Lipay, Monica Vanucci
[5]
;
Ferreira, Lydia Masako
[6]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo. EPM
[2] Univ Fed Sao Paulo. EPM
[3] Univ Fed Sao Paulo. EPM
[4] Univ Fed Sao Paulo. EPM
[5] Univ Fed Sao Paulo. EPM
[6] Univ Fed Sao Paulo. EPM
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Acta Cirurgica Brasileira; v. 27, n. 12, p. 855-860, DEC 2012. |
| Web of Science Citations: | 1 |
| Abstract | |
PURPOSE: To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS: Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS: After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION: The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation. (AU) | |