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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular biology in tropical dermatoses

Full text
Author(s):
Ana Maria Roselino [1]
Total Authors: 1
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Clínica Médica - Brasil
Total Affiliations: 1
Document type: Journal article
Source: ANAIS BRASILEIROS DE DERMATOLOGIA; v. 83, n. 3, p. 187-203, 2008-06-00.
Abstract

Initially, basic concepts are presented concerning the cell, genetic code and protein synthesis, and some techniques of molecular biology, such as PCR, PCR-RFLP, DNA sequencing, RT-PCR and immunoblotting. Protocols of nucleotides and of proteins extraction are supplied, such as salting out in peripheral blood allied to phenol-chloroform and trizol methods in skin samples. To proceed, commented examples of application of those techniques of molecular biology for the etiologic diagnosis and for research in tropical dermatoses, with emphasis to American tegumentary leishmaniasis and leprosy are presented. (AU)