Oligonucleotide primers for specific detection of actinobacterial laccases from superfamilies I and K

Author(s)

Rigamonte Fernandes, Tatiana Alves ^{[1, 2]} ; da Silveira, Wendel Batista ^[2] ; Lopes Passos, Flavia Maria ^[2] ; Zucchi, Tiago Domingues ^[1]

Affiliation

^[1] EMBRAPA Meio Ambiente, Lab Microbiol Ambiental, BR-13820000 Jaguariuna, SP - Brazil

^[2] Univ Fed Vicosa, Dept Microbiol, BIOAGRO, BR-36570900 Vicosa, MG - Brazil

• Document type: Journal article
• Source: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 106, n. 2, p. 391-398, AUG 2014.
• Web of Science Citations: 0

Abstract

Although many putative laccase-like genes have been assigned to members of the phylum Actinobacteria, few of the related enzymes have been characterized so far. It is noteworthy, however, that this small number of enzymes has presented properties with industrial relevance. This observation, combined with the recognized biotechnological potential and the capability of this phylum to degrade recalcitrant soil polymers, has attracted attention for bioprospective approaches. In the present work, we have designed and tested primers that were specific for detection of sub-groups of laccase-like genes within actinomycetes, which corresponded to the superfamilies I and K from the classification presented by the laccase and multicopper oxidase engineering database. The designed primers have amplified laccase-like gene fragments from actinomycete isolates that were undetectable by primers available from the literature. Furthermore, phylogenetic alignments suggest that some of these fragments may belong to new laccases-like proteins, and thus emphasize the benefits of designing subgroup-specific primers. (AU)

• FAPESP's process: 11/14333-6 - Diversity and polyphasic taxonomy of actinobacteria isolated from reforestation areas
• Grantee: Tiago Domingues Zucchi
• Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers