Up-regulation of DNAM-1 and NKp30, associated with improvement of NK cells activation after long-term culture of mononuclear cells from patients with ovarian neoplasia

Author(s)

da Silva, Rodrigo Fernandes ^[1] ; Petta, Carlos Alberto ^{[1, 2]} ; Derchain, Sophie Francoise ^{[1, 2]} ; Alici, Evren ^[3] ; Guimaraes, Fernando ^[1]

Affiliation

^[1] Univ Estadual Campinas, Hosp Mulher Prof Doutor Jose Aristodemo Pinotti, Ctr Atencao Integral & Saude Mulher, UNICAMP, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Tocoginecol, Fac Ciencias Med, UNICAMP, Campinas, SP - Brazil

^[3] Karolinska Univ Hosp, Karolinska Inst, Dept Med, Stockholm - Sweden

• Document type: Journal article
• Source: HUMAN IMMUNOLOGY; v. 75, n. 8, p. 777-784, AUG 2014.
• Web of Science Citations: 6

Abstract

This study aimed at evaluating the functional activation and activating receptors expression on resting, short- and long-term NI( and NK-like T cells from blood of ovarian neoplasia patients. Blood from patients with adnexal benign alterations (n = 10) and ovarian cancer (grade I-IV n = 14) were collected after signed consent. Effector cells activation was evaluated by the expression of the CD107a molecule. Short-term culture was conducted overnight with IL-2 and long-term culture for 21 days, by a method designed to expand CD56(+) lymphocytes. Short-term culture significantly increased NK cells activation compared to resting NK cells (p < 0.05), however, the long-term procedure supported an even higher increase (p < 0.001). Resting NK-like T cells showed poor activation, which was not altered by the culture procedures. The long-term culture effectively increased the expression of the activating receptors on NK and NK-like T cells, either by increasing the number of cells expressing a given receptor and/or by up-regulating their expression intensity. As a conclusion, the long-term culture system employed, resulted in a high number of functional NK cells. The culture system was particularly efficient on the up-regulation of NKp30 and DNAM-1 receptors on NK cells. (C) 2014 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 11/14520-0 - Natural Killer cell-mediated targeting of human ovarian cancer and possibilities for immunotherapy
• Grantee: Fernando Guimarães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/17202-7 - Autologous antitumor activity of NK cells expanded in vitro from ovarian cancer patients
• Grantee: Rodrigo Fernandes da Silva
• Support Opportunities: Scholarships in Brazil - Master