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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combining Experimental Evidence and Molecular Dynamic Simulations To Understand the Mechanism of Action of the Antimicrobial Octapeptide Jelleine-I

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dos Santos Cabrera, Marcia Perez [1, 2] ; Baldissera, Gisele [1, 3] ; Silva-Goncalves, Laiz da Costa [4] ; de Souza, Bibiana Monson [5] ; Riske, Karin A. [4] ; Palma, Mario Sergio [5] ; Ruggiero, Jose Roberto [1] ; Arcisio-Miranda, Manoel [4]
Total Authors: 8
[1] Univ Estadual Paulista, Dept Fis, BR-15054000 Sao Paulo - Brazil
[2] Univ Estadual Paulista, Dept Quim & Ciencias Ambientais, BR-15054000 Sao Paulo - Brazil
[3] Fac Tecnol Catanduva, BR-15800200 Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Biofis, BR-04023062 Sao Paulo - Brazil
[5] Univ Estadual Paulista, Ctr Estudos Insetos Sociais, BR-13506900 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: BIOCHEMISTRY; v. 53, n. 29, p. 4857-4868, JUL 29 2014.
Web of Science Citations: 8

Jelleines are four naturally occurring peptides that comprise approximately eight or nine C-terminal residues in the sequence of the major royal jelly protein 1 precursor (Apis mellifera). The difference between these peptides is limited to one residue in the sequence, but this residue has a significant impact in their efficacy as antimicrobials. In peptidebilayer experiments, we demonstrated that the lytic, poreforming activity of Jelleine-I is similar to that of other cationic antimicrobial peptides, which exhibit stronger activity on anionic bilayers. Results from molecular dynamics simulations suggested that the presence of a proline residue at the first position is the underlying reason for the higher efficacy of Jelleine-I compared with the other jelleines. Additionally, simulations suggested that Jelleine-I tends to form aggregates in water and in the presence of mimetic membrane environments. Combined experimental evidence and simulations showed that the protonation of the histidine residue potentiates the interaction with anionic palmitoyloleoyl-phosphatidylcholine/palmitoyl-oleoyl-phosphatidylglycero l (POPC/POPG) (70:30) bilayers and reduces the free energy barrier for water passage. The interaction is driven by electrostatic interactions with the headgroup region of the bilayer with some disturbance of the acyl chain region. Our findings point to a mechanism of action by which aggregated Jelleine-I accumulates on the headgroup region of the membrane. Remaining in this form, Jelleine-I could exert pressure to accommodate its polar and nonpolar residues on the amphiphilic environment of the membrane. This pressure could open pores or defects, could disturb the bilayer continuity, and leakage would be observed. The agreement between experimental data and simulations in mimetic membranes suggests that this approach may be a valuable tool to the understanding of the molecular mechanisms of action. (AU)

FAPESP's process: 06/57122-7 - Searching for lead compounds for rational development of new drugs and pesticides through bioprospecting in Brazilian arthropods
Grantee:Mario Sergio Palma
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 10/52077-9 - Molecular aspects of the voltage sensor of ionic channels: structure, kinetics and evolution
Grantee:Manoel de Arcisio Miranda Filho
Support type: Regular Research Grants
FAPESP's process: 10/11823-0 - Mechanisms of interactions of lipid bilayers and amphiphilic substances of therapeutic interest
Grantee:Marcia Perez dos Santos Cabrera
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/24259-0 - Peptide and chitosan conjugates with pharmacological potential: synthesis, prospecting of activity in membrane mimetic systems, and evaluation in cells
Grantee:Marcia Perez dos Santos Cabrera
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 11/51684-1 - System biology as experimental strategy for discovery of novel natural products in the fauna of venomous arthropods from São Paulo State
Grantee:Mario Sergio Palma
Support type: BIOTA-FAPESP Program - Thematic Grants