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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CROSSOVER CLINICAL TRIAL OF THE INFLUENCE OF THE USE OF ADHESIVE ON BIOFILM FORMATION

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Author(s):
Leite, Andressa R. P. [1] ; Mendoza-Marin, Danny O. [1] ; Paleari, Andre G. [1] ; Rodriguez, Larissa S. [1] ; Roccia, Andreia A. [1] ; Policastro, Vivian B. [1] ; Compagnoni, Marco A. [1] ; de Souza, Raphael F. [2] ; Pero, Ana C. [1]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Sao Paulo - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Dent Sch, Dept Dent Mat & Prosthodont, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF PROSTHETIC DENTISTRY; v. 112, n. 2, p. 349-356, AUG 2014.
Web of Science Citations: 12
Abstract

Statement of problem. Contrasting results have been reported regarding the influence of the use of adhesive on biofilm formation. Purpose. The purpose of this study was to evaluate the influence of the use of adhesive on the formation of biofilm on the internal surface of complete dentures and the palatal mucosa of denture wearers. Material and methods. Thirty participants with well-fitting complete dentures were randomly divided according to the experimental design: protocol 1, adhesive use during the first 15 days, followed by no use of adhesive over the next 15 days; protocol 2, no use of adhesives during the first 15 days, followed by adhesive use over the next 15 days. After each period, material from the mucosa and intaglio of the maxillary dentures was collected. Replicate aliquots were plated onto Petri dishes containing selective media for Candida spp, Streptococcus mutans, and a nonselective culture medium. Colony-forming units were expressed as log (CFU+1)/mL. In addition, the internal surfaces of the maxillary and mandibular complete dentures were stained and photographed. From the photographs, the total internal surface and the surface stained with biofilm were quantified (software ImageTool 3.00), and the percentage of the biofilm-covered area (%) on the maxillary and mandibular dentures was calculated and compared with 2-way ANOVA. For the nonselective culture medium, data were compared with the paired-sample t test, and the Wilcoxon signed rank test was performed to compare the colony counts of Candida spp and Streptococcus mutans (alpha=.05). Results. Similar colony counts were found with or without the use of adhesive for the mucosa and internal surfaces of maxillary dentures, irrespective of the culture medium (P>.05). The area of dentures covered with biofilm was influenced by the use of adhesive (P=.025), regardless of the type of denture (P=.121). Conclusions. The use of adhesive did not alter the colony counts of microorganisms from the palatal mucosa and maxillary dentures of complete denture wearers during the 15-day period, but it did influence the area covered with biofilm on the internal surfaces of the complete dentures. (AU)

FAPESP's process: 10/00736-9 - Effect of using an adhesive in conventional complete denture wearers
Grantee:Marco Antonio Compagnoni
Support Opportunities: Regular Research Grants
FAPESP's process: 11/01320-3 - Evaluation of aspects related to the use of an adhesive in complete denture wearers
Grantee:Andressa Rosa Perin Leite
Support Opportunities: Scholarships in Brazil - Master