Changes in calsequestrin, TNF-alpha, TGF-beta and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles

Autor(es)

Maranhao, Juliana Barros ^[1] ; Moreira, Drielen de Oliveira ^[2] ; Mauricio, Adriana Fogagnolo ^[2] ; de Carvalho, Samara Camacari ^[2] ; Ferretti, Renato ^[3] ; Pereira, Juliano Alves ^[2] ; Santo Neto, Humberto ^[2] ; Marques, Maria Julia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Pernambuco UPE, Recife, PE - Brazil

^[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Estrut & Func, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Experimental Pathology; v. 96, n. 5, p. 285-293, OCT 2015.
• Citações Web of Science: 7

Resumo

In Duchenne muscular dystrophy (DMD), the search for new biomarkers to follow the evolution of the disease is of fundamental importance in the light of the evolving gene and pharmacological therapies. In addition to the lack of dystrophin, secondary events including changes in calcium levels, inflammation and fibrosis greatly contribute to DMD progression and the molecules involved in these events may represent potential biomarkers. In this study, we performed a comparative evaluation of the progression of dystrophy within muscles that are differently affected by dystrophy (diaphragm; DIA and quadriceps; QDR) or spared (intrinsic laryngeal muscles) using the mdx mice model of DMD. We assessed muscle levels of calsequestrin (calcium-related protein), tumour necrosis factor (TNF-alpha; pro-inflammatory cytokine), tumour growth factor (TGF-beta; pro-fibrotic factor) and MyoD (muscle proliferation) vs. histopathology at early (1 and 4 months of age) and late (9 months of age) stages of dystrophy. Fibrosis was the primary feature in the DIA of mdx mice (9 months: 32% fibrosis), which was greater than in the QDR (9 months: 0.6% fibrosis). Muscle regeneration was the primary feature in the QDR (9 months: 90% of centrally nucleated fibres areas vs. 33% in the DIA). The QDR expressed higher levels of calsequestrin than the DIA. Laryngeal muscles showed normal levels of TNF-alpha, TGF-beta and MyoD. A positive correlation between histopathology and cytokine levels was observed only in the diaphragm, suggesting that TNF-alpha and TGF-beta serve as markers of dystrophy primarily for the diaphragm. (AU)

• Processo FAPESP: 12/03498-7 - Mecanismos de ação da suramina na cardiomiopatia do camundongo mdx
• Beneficiário: Drielen de Oliveira Moreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/15526-9 - Proteínas reguladoras do cálcio e proteção à mionecrose na distrofia muscular de Duchenne
• Beneficiário: Maria Julia Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58491-1 - Mecanismos de proteção da distrofia muscular: estudo proteômico e terapia farmacológica
• Beneficiário: Maria Julia Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/51697-6 - Mecanismos de acao do acido eicosapentanoico (epa) na protecao a mionecrose em fibras musculares distroficas
• Beneficiário: Maria Julia Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/13577-1 - Biomarcadores da cardiomiopatia para distrofia muscular: estudo metabolômico e terapia farmacológica
• Beneficiário: Adriana Fogagnolo Mauricio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/15492-3 - Terapia farmacológica das distrofinopatias: fibrose e regeneração muscular em camundongos mdx tratados com ômega-3
• Beneficiário: Samara Camaçarí de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/04782-6 - Estudos pré-clínicos no camundongo mdx: metabolômica, biomarcadores e terapia com ômega-3
• Beneficiário: Maria Julia Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular