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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Changes in calsequestrin, TNF-alpha, TGF-beta and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles

Texto completo
Autor(es):
Maranhao, Juliana Barros [1] ; Moreira, Drielen de Oliveira [2] ; Mauricio, Adriana Fogagnolo [2] ; de Carvalho, Samara Camacari [2] ; Ferretti, Renato [3] ; Pereira, Juliano Alves [2] ; Santo Neto, Humberto [2] ; Marques, Maria Julia [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Pernambuco UPE, Recife, PE - Brazil
[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Estrut & Func, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Anat, Botucatu, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: International Journal of Experimental Pathology; v. 96, n. 5, p. 285-293, OCT 2015.
Citações Web of Science: 7
Resumo

In Duchenne muscular dystrophy (DMD), the search for new biomarkers to follow the evolution of the disease is of fundamental importance in the light of the evolving gene and pharmacological therapies. In addition to the lack of dystrophin, secondary events including changes in calcium levels, inflammation and fibrosis greatly contribute to DMD progression and the molecules involved in these events may represent potential biomarkers. In this study, we performed a comparative evaluation of the progression of dystrophy within muscles that are differently affected by dystrophy (diaphragm; DIA and quadriceps; QDR) or spared (intrinsic laryngeal muscles) using the mdx mice model of DMD. We assessed muscle levels of calsequestrin (calcium-related protein), tumour necrosis factor (TNF-alpha; pro-inflammatory cytokine), tumour growth factor (TGF-beta; pro-fibrotic factor) and MyoD (muscle proliferation) vs. histopathology at early (1 and 4 months of age) and late (9 months of age) stages of dystrophy. Fibrosis was the primary feature in the DIA of mdx mice (9 months: 32% fibrosis), which was greater than in the QDR (9 months: 0.6% fibrosis). Muscle regeneration was the primary feature in the QDR (9 months: 90% of centrally nucleated fibres areas vs. 33% in the DIA). The QDR expressed higher levels of calsequestrin than the DIA. Laryngeal muscles showed normal levels of TNF-alpha, TGF-beta and MyoD. A positive correlation between histopathology and cytokine levels was observed only in the diaphragm, suggesting that TNF-alpha and TGF-beta serve as markers of dystrophy primarily for the diaphragm. (AU)

Processo FAPESP: 11/51697-6 - Mecanismos de ação do ácido eicosapentanóico (EPA) na proteção a mionecrose em fibras musculares distróficas
Beneficiário:Maria Julia Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/58491-1 - Mecanismos de proteção da distrofia muscular: estudo proteômico e terapia farmacológica
Beneficiário:Maria Julia Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/03498-7 - Mecanismos de ação da suramina na cardiomiopatia do camundongo mdx
Beneficiário:Drielen de Oliveira Moreira
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 04/15526-9 - Proteínas reguladoras do cálcio e proteção à mionecrose na distrofia muscular de Duchenne
Beneficiário:Maria Julia Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/04782-6 - Estudos pré-clínicos no camundongo mdx: metabolômica, biomarcadores e terapia com ômega-3
Beneficiário:Maria Julia Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/13577-1 - Biomarcadores da cardiomiopatia para distrofia muscular: estudo metabolômico e terapia farmacológica
Beneficiário:Adriana Fogagnolo Mauricio
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/15492-3 - Terapia farmacológica das distrofinopatias: fibrose e regeneração muscular em camundongos mdx tratados com ômega-3
Beneficiário:Samara Camaçarí de Carvalho
Linha de fomento: Bolsas no Brasil - Doutorado