Cancer stem cell, cytokeratins and epithelial to mesenchymal transition markers expression in oral squamous cell carcinoma derived from ortothopic xenoimplantation of CD44(high) cells

Autor(es)

de Andrade, Nathalia Paiva ; Setubal Destro Rodrigues, Maria Fernanda ; Rodini, Camila Oliveira ; Nunes, Fabio Daumas

• Tipo de documento: Artigo Científico
• Fonte: PATHOLOGY RESEARCH AND PRACTICE; v. 213, n. 3, p. 235-244, 2017.
• Citações Web of Science: 11

Resumo

Oral squamous cell carcinoma (OSCC) is the most prevalent neoplasia of oral cavity worldwide and prognosis remains unchanged in decades. Recently, different authors reported that head and neck squamous cell carcinomas have a subpopulation of tumor initiating cells that apparently correspond to cancer stem cells (CSC) and are also responsible for tumor growth and metastasis. The purpose of the present study was to investigate the microscopic and phenotypic characteristics of OSCC tumors induced after orthotopic xenoimplantation of SCC9(WT) cell line and CSC-enriched subpopulation isolated from SCC9 cell line based on high expression of the putative CSC marker CD44. Different numbers of FACS-sorted SCC9 CD44(high) and CD44(low) cells as well as SCC9(WT) (wild type) were transplanted into the tongue of BALB/C nude (NOD/SCID) mice to evaluate their tumorigenic potential. Sixty days post-induction, tumors were morphologically characterized and immunostained for CSC markers (CD44, Nanog and Bmi-1), epithelial-mesenchymal transition (Snail, Slug) and epithelial differentiating cell markers (cytokeratins 4, 13, 15, 17 and 19), as well as E-cadherin and p-catenin. The data presented here shows that SCC9 CD44(high c)ells have higher ability to form tumors than SCC9 CD44I0w cells, even when significantly lower numbers of SCC9 CD44(high) cells were transplanted. Immunoassessment of tumors derived from SCC9 CD44(high) cells revealed high expression of cytokeratin CK19, beta-catenin, E-cadherin and CD44, and negative or low expression of CK17, CK4, CK15, CK13, Nanog, Bmi-1, Snail and Slug. While tumors derived from SCC9 T showed high expression of CK17, CK19, CD44, Nanog, Bmi-1, Snail and Slug, and negative or low expression of CK4, CK15, CK13, beta-catenin and E-cadherin. Thus, SCC9 CD44(high) cells were highly tumorigenic, capable of originating heterogeneous tumors and these tumors have a immunohistochemical profile different from those formed by the wild type cell line. (C) 2016 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 12/00786-1 - Análise funcional da participação de vias de sinalização em células-tronco tumorais isoladas de linhagens celulares derivadas de carcinoma epidermóide bucal
• Beneficiário: Fabio Daumas Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/20622-3 - Caracterização de células tronco tumorais após inoculação em camundongos nude
• Beneficiário: Nathália Paiva de Andrade
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/21395-8 - Análise funcional da participação de vias de sinalização em células-tronco tumorais isoladas de linhagens celulares derivadas de carcinoma epidermóide bucal.
• Beneficiário: Maria Fernanda Setúbal Destro Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado