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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cancer stem cell, cytokeratins and epithelial to mesenchymal transition markers expression in oral squamous cell carcinoma derived from ortothopic xenoimplantation of CD44(high) cells

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de Andrade, Nathalia Paiva ; Setubal Destro Rodrigues, Maria Fernanda ; Rodini, Camila Oliveira ; Nunes, Fabio Daumas
Total Authors: 4
Document type: Journal article
Source: PATHOLOGY RESEARCH AND PRACTICE; v. 213, n. 3, p. 235-244, 2017.
Web of Science Citations: 11

Oral squamous cell carcinoma (OSCC) is the most prevalent neoplasia of oral cavity worldwide and prognosis remains unchanged in decades. Recently, different authors reported that head and neck squamous cell carcinomas have a subpopulation of tumor initiating cells that apparently correspond to cancer stem cells (CSC) and are also responsible for tumor growth and metastasis. The purpose of the present study was to investigate the microscopic and phenotypic characteristics of OSCC tumors induced after orthotopic xenoimplantation of SCC9(WT) cell line and CSC-enriched subpopulation isolated from SCC9 cell line based on high expression of the putative CSC marker CD44. Different numbers of FACS-sorted SCC9 CD44(high) and CD44(low) cells as well as SCC9(WT) (wild type) were transplanted into the tongue of BALB/C nude (NOD/SCID) mice to evaluate their tumorigenic potential. Sixty days post-induction, tumors were morphologically characterized and immunostained for CSC markers (CD44, Nanog and Bmi-1), epithelial-mesenchymal transition (Snail, Slug) and epithelial differentiating cell markers (cytokeratins 4, 13, 15, 17 and 19), as well as E-cadherin and p-catenin. The data presented here shows that SCC9 CD44(high c)ells have higher ability to form tumors than SCC9 CD44I0w cells, even when significantly lower numbers of SCC9 CD44(high) cells were transplanted. Immunoassessment of tumors derived from SCC9 CD44(high) cells revealed high expression of cytokeratin CK19, beta-catenin, E-cadherin and CD44, and negative or low expression of CK17, CK4, CK15, CK13, Nanog, Bmi-1, Snail and Slug. While tumors derived from SCC9 T showed high expression of CK17, CK19, CD44, Nanog, Bmi-1, Snail and Slug, and negative or low expression of CK4, CK15, CK13, beta-catenin and E-cadherin. Thus, SCC9 CD44(high) cells were highly tumorigenic, capable of originating heterogeneous tumors and these tumors have a immunohistochemical profile different from those formed by the wild type cell line. (C) 2016 Elsevier GmbH. All rights reserved. (AU)

FAPESP's process: 12/00786-1 - Functional analysis of cell signaling involvement in cancer stem cells obtained from oral squamous cell carcinoma cell lines
Grantee:Fabio Daumas Nunes
Support type: Regular Research Grants
FAPESP's process: 12/20622-3 - Characterization of tumor stem cells after inoculation in nude mice
Grantee:Nathália Paiva de Andrade
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/21395-8 - Functional analysis of cell signaling involvement in cancer stem cells obtained from oral squamous cell carcinoma cell lines
Grantee:Maria Fernanda Setúbal Destro Rodrigues
Support type: Scholarships in Brazil - Post-Doctorate