Twenty-four Weeks of beta-Alanine Supplementation on Carnosine Content, Related Genes, and Exercise

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Saunders, Bryan ; Painelli, Vitor De Salles ; De Oliveira, Luana Farias ; Silva, Vinicius Da Eira ; Da Silva, Rafael Pires ; Riani, Luiz ; Franchi, Mariana ; Goncalves, Livia De Souza ; Harris, Roger Charles ; Roschel, Hamilton ; Artioli, Guilherme Giannini ; Sale, Craig ; Gualano, Bruno

• Tipo de documento: Artigo Científico
• Fonte: MEDICINE AND SCIENCE IN SPORTS AND EXERCISE; v. 49, n. 5, p. 896-906, MAY 2017.
• Citações Web of Science: 25

Resumo

Introduction: Skeletal muscle carnosine content can be increased through beta-alanine (BA) supplementation, but the maximum increase achievable with supplementation is unknown. No study has investigated the effects of prolonged supplementation on carnosine-related genes or exercise capacity. Purpose: This study aimed to investigate the effects of 24 wk of BA supplementation on muscle carnosine content, gene expression, and high-intensity cycling capacity (CCT110%). Methods: Twenty-five active males were supplemented with 6.4 gId(-1) of sustained release BA or placebo for a 24 wk period. Every 4 wk participants provided a muscle biopsy and performed the CCT110%. Biopsies were analyzed for muscle carnosine content and gene expression (CARNS, TauT, ABAT, CNDP2, PHT1, PEPT2, and PAT1). Results: Carnosine content was increased from baseline at every time point in BA (all P < 0.0001; week 4 = + 11.37 +/- 7.03 mmolIkg(-1) dm, week 8 = + 13.88 +/- 7.84 mmolIkg(-1) dm, week 12 = + 16.95 +/- 8.54 mmolIkg(-1) dm, week 16 = + 17.63 +/- 8.42 mmolIkg(-1) dm, week 20 = + 21.20 +/- 7.86 mmolIkg (-1) dm, and week 24 = + 20.15 +/- 7.63 mmolIkg (-1) dm) but not placebo (all P < 0.05). Maximal increases were + 25.66 +/- 7.63 mmolIkg(-1) dm (range = + 17.13 to + 41.32 mmolIkg(-1) dm), and absolute maximal content was 48.03 +/- 8.97 mmolIkg(-1) dm (range = 31.79 to 63.92 mmolIkg(-1) dm). There was an effect of supplement (P = 0.002) on TauT; no further differences in gene expression were shown. Exercise capacity was improved in BA (P = 0.05) with possible to almost certain improvements across all weeks. Conclusions: Twenty-four weeks of BA supplementation increased muscle carnosine content and improved high-intensity cycling capacity. The downregulation of TauT suggests it plays an important role in muscle carnosine accumulation with BA supplementation, whereas the variability in changes in muscle carnosine content between individuals suggests that other determinants other than the availability of BA may also bear a major influence on muscle carnosine content. (AU)

• Processo FAPESP: 13/14746-4 - Metabolismo da carnosina no músculo esquelético: um estudo de múltiplas abordagens
• Beneficiário: Bruno Gualano
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/11948-8 - Vida sem carnosina: desenvolvimento e caracterização de um modelo de rato nocaute para estudo do papel fisiológico da carnosina e suas implicações para o exercício físico e metabolismo muscular
• Beneficiário: Guilherme Giannini Artioli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/13026-5 - Suplementação de beta-alanina e bicarbonato de sódio: efeitos sobre a utilização dos sistemas energéticos durante o exercício anaeróbio intermitente
• Beneficiário: Rafael Pires da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/19513-2 - Efeitos da Suplementação Crônica de Beta-Alanina Combinada ou não à Ingestão Aguda de Bicarbonato de Sódio sobre a Concentração Pico de Carnosina, o "Washout" de Carnosina e o Desempenho em Exercício Intenso.
• Beneficiário: Bryan Saunders
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/04806-0 - Efeitos de 12 semanas de treinamento intermitente de alta intensidade sobre as concentrações intramusculares de carnosina
• Beneficiário: Vitor de Salles Painelli
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/22686-7 - Efeito de seis meses de suplementação de beta-alanina sobre o conteúdo intramuscular de carnosina e taurina
• Beneficiário: Mariana Franchi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica