Selenium Bioaccessibility and Speciation in Selenium-Enriched Lettuce: Investigation of the Selenocompounds Liberated after in Vitro Simulated Human Digestion Using Two-Dimensional HPLC-ICP-MS

Autor(es)

da Silva, Emanueli do Nascimento ; Aureli, Federica ; D'Amato, Marilena ; Raggi, Andrea ; Cadore, Solange ; Cubadda, Francesco

• Tipo de documento: Artigo Científico
• Fonte: Journal of Agricultural and Food Chemistry; v. 65, n. 14, p. 3031-3038, APR 12 2017.
• Citações Web of Science: 15

Resumo

The evaluation of selenium-enriched vegetables as potential dietary sources of selenium, an essential element for humans, requires an assessment of the plant's accumulation ability as well as of the bioaccessibility and speciation of the accumulated selenium, which influence its biological effects in humans. Lettuce hydroponically grown at three selenite (SeVI)/selenate (SeIV) amendment levels was characterized accordingly. Selenium accumulation in lettuce leaves was greatest with Se(VI) amendment, whereas bioaccessibility was 70% on average in both cases. Selenium speciation in gastrointestinal hydrolysates, characterized by anion and cation exchange HPLC-ICP-MS, showed that Se(IV) was largely biotransformed into organoselenium metabolites, with selenomethionine accounting for 1/3 of the total detected species, whereas Se(VI) was incorporated as such in the edible portion of the plant, with only a small fraction (similar to,20%) converted into organic species. Taking into account both nutritional quality and safety, the Se(IV)-enriched lettuce appeared more favorable as a potential selenium source for human consumption. (AU)

• Processo FAPESP: 12/15020-4 - Bioacessibilidade e biodisponibilidade de nutrientes e elementos tóxicos em alface (Lactuca sativa)
• Beneficiário: Emanueli Do Nascimento da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/15425-0 - Biofortificação de selênio em alface: estudo de bioacessibilidade e especiação de selênio na parte comestível da planta
• Beneficiário: Emanueli Do Nascimento da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/26855-2 - Avaliação da fração bioacessível e da biodisponibilidade de nutrientes e contaminantes em hortaliças
• Beneficiário: Solange Cadore
• Modalidade de apoio: Auxílio à Pesquisa - Regular