Distinctive role of efferocytosis in dendritic cell maturation and migration in sterile or infectious conditions

Autor(es)

Penteado, Leticia de Aquino ; Dejani, Naiara Naiana ; Verdan, Felipe Fortino ; Orlando, Allan Botinhon ; Nino, Victoria Eugenia ; Dias, Fernanda De Nuzzi ; Guerta Salina, Ana Carolina ; Medeiros, Alexandra Ivo

• Tipo de documento: Artigo Científico
• Fonte: IMMUNOLOGY; v. 151, n. 3, p. 304-313, JUL 2017.
• Citações Web of Science: 5

Resumo

Efferocytosis, or clearance of apoptotic cells (ACs), by dendritic cells (DCs) leads to immune response suppression and tolerance to self-antigens. However, efferocytosis of infected apoptotic cells (IACs) leads to the production of a mixed pro- and anti-inflammatory cytokine milieu. We examined the DC phenotype and ability to migrate after phagocytosis of ACs or IACs and observed higher levels of CD86 and CCR7 expression in DCs, as well as enhanced migration capacity following efferocytosis of IACs. Interestingly, higher levels of interleukin-1, interleukin-10 and prostaglandin E-2 (PGE(2)) were also produced in this context. Blockage of IAC recognition led to an impaired maturation profile and PGE(2) production, which may have contributed to reduced CD86 and CCR7 expression and migration capacity. These data contribute to the understanding of how efferocytosis of sterile or infected cells may regulate the adaptive immune response, although the precise role of PGE(2) in this process requires further investigation. (AU)

• Processo FAPESP: 16/10964-5 - Mecanismo pelo qual PGE2 oriunda da eferocitose inibe a expressão do IL-1R e a diferenciação de Th17 - Envolvimento de SOCS3
• Beneficiário: Allan Botinhon Orlando
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/17611-7 - Efeito da fagocitose de células apoptóticas por células dendríticas na diferenciação de células Th17: função de PGE2
• Beneficiário: Alexandra Ivo de Medeiros
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/23580-0 - Diferenciação de células Th17 durante a fagocitose de células apoptóticas infectadas: determinação das vias de sinalização intracelular via PGE2 e receptores EP
• Beneficiário: Naiara Naiana Dejani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/03967-2 - Perfil fenotípico e capacidade migratória de células dendríticas após fagocitose de células apoptóticas
• Beneficiário: Letícia de Aquino Penteado
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica