Distinctive role of efferocytosis in dendritic cell maturation and migration in sterile or infectious conditions

Author(s)

Penteado, Leticia de Aquino ; Dejani, Naiara Naiana ; Verdan, Felipe Fortino ; Orlando, Allan Botinhon ; Nino, Victoria Eugenia ; Dias, Fernanda De Nuzzi ; Guerta Salina, Ana Carolina ; Medeiros, Alexandra Ivo

• Document type: Journal article
• Source: IMMUNOLOGY; v. 151, n. 3, p. 304-313, JUL 2017.
• Web of Science Citations: 5

Abstract

Efferocytosis, or clearance of apoptotic cells (ACs), by dendritic cells (DCs) leads to immune response suppression and tolerance to self-antigens. However, efferocytosis of infected apoptotic cells (IACs) leads to the production of a mixed pro- and anti-inflammatory cytokine milieu. We examined the DC phenotype and ability to migrate after phagocytosis of ACs or IACs and observed higher levels of CD86 and CCR7 expression in DCs, as well as enhanced migration capacity following efferocytosis of IACs. Interestingly, higher levels of interleukin-1, interleukin-10 and prostaglandin E-2 (PGE(2)) were also produced in this context. Blockage of IAC recognition led to an impaired maturation profile and PGE(2) production, which may have contributed to reduced CD86 and CCR7 expression and migration capacity. These data contribute to the understanding of how efferocytosis of sterile or infected cells may regulate the adaptive immune response, although the precise role of PGE(2) in this process requires further investigation. (AU)

• FAPESP's process: 16/10964-5 - Mechanism by which PGE2 originated from efferocytosis inhibits the expression of Il-1R and Th17 differentiation - Involvement of SOCS3
• Grantee: Allan Botinhon Orlando
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/17611-7 - Effect of efferocytosis by dendritic cells on Th17 differentiation: role of Prostaglandin E2
• Grantee: Alexandra Ivo de Medeiros
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 12/23580-0 - Differentiation of Th17 cells during phagocytosis of infected apoptotic cells: Determination of intracellular signaling pathways via PGE2 and EP receptors
• Grantee: Naiara Naiana Dejani
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/03967-2 - Phenotypic profile and migratory capacity of dendritic cells after apoptotic cell phagocytosis
• Grantee: Letícia de Aquino Penteado
• Support Opportunities: Scholarships in Brazil - Scientific Initiation