Paracoccin distribution supports its role in Paracoccidioides brasiliensis growth and dimorphic transformation

Autor(es)

Oliveira, Aline Ferreira ; Fernandes, Fabricio Freitas ; Mariano, Vania Sammartino ; Almeida, Fausto ; Ruas, Luciana Pereira ; Oliveira, Leandro Licursi ; Oliver, Constance ; Jamur, Maria Celia ; Roque-Barreira, Maria Cristina

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 12, n. 8 AUG 28 2017.
• Citações Web of Science: 1

Resumo

Paracoccidioides brasiliensis yeast was reported to express paracoccin, a GlcNAc-binding protein that displays N-acetyl-beta-D-glucosaminidase (NAGase) activity. Highly specific antiparacoccin antibodies have been previously used to examine the localization of paracoccin in yeast and inhibit its growth in vitro. In the present study, anti-paracoccin antibodies were used to characterize, by scanning confocal microscopy, the distribution of paracoccin in P. brasiliensis hyphae, transition forms from hyphae to yeast, and mature yeast. In the mycelial phase, paracoccin was detected mainly in the hyphae tips, where it demonstrated a punctate distribution, and was associated with the cell wall. During the first 48 hours after a temperature shift from 26 degrees C to 37 degrees C, paracoccin expression in the differentiating hyphae was mainly detected in the budding regions, i.e. lateral protrusions, and inside the new daughter cells. There was an increased number of chlamydoconidia that expressed a high concentration of paracoccin on their surfaces and/or in their interiors 72-96 hours after the temperature shift. After 120 hours, yeast cells were the predominant form and their cytoplasm stained extensively for paracoccin, whereas Wheat Germ Agglutinin (WGA) staining was predominant on their exterior walls. After 10 days at 37 degrees C, the interior of both mother and daughter yeast cells, as well as the budding regions, stained intensely for paracoccin. The comparison of mRNA-expression in the different fungal forms showed that PCN transcripts, although detected in all evaluated morphological forms, were higher in hypha and yeast-tohypha transition forms. In conclusion, the pattern of paracoccin distribution in all P. brasiliensis morphotypes supports prevalent beliefs that it plays important roles in fungal growth and dimorphic transformation. (AU)

• Processo FAPESP: 13/10741-8 - Efeito da inibição de N-glicosilação sobre o perfil transcricional e proteômico de Paracoccidioides brasiliensis
• Beneficiário: Fausto Bruno dos Reis Almeida
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/58879-7 - Reconhecimento de padroes moleculares de paracoccidioides brasiliensis e sua participacao na ativacao de fagocitos induzida por km+.
• Beneficiário: Vânia Sammartino Mariano
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/60642-2 - Efeitos biológicos e aplicações farmacêuticas de lectinas
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/51197-3 - Papel de N-glicanas em atividades desempenhadas por paracoccina
• Beneficiário: Fausto Bruno dos Reis Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado