Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?

Autor(es):Mostrar menos -

de Morais-Zani, Karen ^{[1, 2]} ; Serino-Silva, Caroline ^{[1, 2]} ; Galizio, Nathalia da Costa ^[1] ; Tasima, Lidia Jorge ^[1] ; Pagotto, Josias Falararo ^[3] ; Teixeira da Rocha, Marisa Maria ^[1] ; Marcelino, Jose Roberto ^[4] ; Sant'Anna, Savio Stefanini ^[1] ; Tashima, Alexandre Keiji ^[3] ; Tanaka-Azevedo, Anita Mitico ^{[1, 2]} ; Grego, Kathleen Fernandes ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Herpetol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Pesquisas Tecnol, Inst Butantan, Interunidades Biotecnol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, Sao Paulo - Brazil

^[4] Inst Butantan, Serv Imunol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 174, p. 17-27, MAR 1 2018.
• Citações Web of Science: 3

Resumo

Considering that the scarcity of venom represents a huge challenge for biochemical and functional studies of Micrurus species (coral snakes), in this report we describe for the first time the influence of pilocarpine administration prior to venom milking on the yield and protein composition of Micrurus corallinus venom. The administration of pilocarpine resulted in an increase of about 127% in the volume of venom milked, with similar protein content. Venoms showed similar protein bands distribution and intensity by SDS-PAGE and equivalents RP-HPLC profiles. Our proteomic analysis showed that venoms milked in the presence and absence of pilocarpine presented comparable protein profiles, in terms of protein composition and relative abundance. The toxins identified were assigned to 13 protein families and represent the most complete M. corallinus venom proteome described so far, in terms of number of protein families identified. Our data indicate that the administration of pilocarpine prior to venom milking increases the venom yield and does not change significantly the venom composition of M. corallinus. The employment of pilocarpine represents a useful approach to increase the yield of venom not only for Micrurus species, but also for other genera of snakes with limitations regarding the amount of venom available. (AU)

• Processo FAPESP: 17/01890-0 - Estudo comparativo da composição do veneno das serpentes Crotalus durissus terrificus nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan e do veneno crotálico de referência nacional
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/11108-0 - Estudo comparativo da composição das biomoléculas dos venenos das serpentes Bothrops jararaca e Crotalus durissus terrificus nascidas e mantidas no Instituto Butantan e recém-chegadas da natureza
• Beneficiário: Karen de Morais Zani
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/19321-9 - Caracterização proteômica e peptidômica dos venenos de aranhas brasileiras por espectrometria de massas
• Beneficiário: Alexandre Keiji Tashima
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/09976-9 - Comparação do veneno de serpentes Bothrops jararaca em cativeiro e recém-chegadas da natureza
• Beneficiário: Nathália da Costa Galizio
• Linha de fomento: Bolsas no Brasil - Iniciação Científica