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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?

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Author(s):
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de Morais-Zani, Karen [1, 2] ; Serino-Silva, Caroline [1, 2] ; Galizio, Nathalia da Costa [1] ; Tasima, Lidia Jorge [1] ; Pagotto, Josias Falararo [3] ; Teixeira da Rocha, Marisa Maria [1] ; Marcelino, Jose Roberto [4] ; Sant'Anna, Savio Stefanini [1] ; Tashima, Alexandre Keiji [3] ; Tanaka-Azevedo, Anita Mitico [1, 2] ; Grego, Kathleen Fernandes [1]
Total Authors: 11
Affiliation:
[1] Inst Butantan, Lab Herpetol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Pesquisas Tecnol, Inst Butantan, Interunidades Biotecnol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, Sao Paulo - Brazil
[4] Inst Butantan, Serv Imunol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF PROTEOMICS; v. 174, p. 17-27, MAR 1 2018.
Web of Science Citations: 3
Abstract

Considering that the scarcity of venom represents a huge challenge for biochemical and functional studies of Micrurus species (coral snakes), in this report we describe for the first time the influence of pilocarpine administration prior to venom milking on the yield and protein composition of Micrurus corallinus venom. The administration of pilocarpine resulted in an increase of about 127% in the volume of venom milked, with similar protein content. Venoms showed similar protein bands distribution and intensity by SDS-PAGE and equivalents RP-HPLC profiles. Our proteomic analysis showed that venoms milked in the presence and absence of pilocarpine presented comparable protein profiles, in terms of protein composition and relative abundance. The toxins identified were assigned to 13 protein families and represent the most complete M. corallinus venom proteome described so far, in terms of number of protein families identified. Our data indicate that the administration of pilocarpine prior to venom milking increases the venom yield and does not change significantly the venom composition of M. corallinus. The employment of pilocarpine represents a useful approach to increase the yield of venom not only for Micrurus species, but also for other genera of snakes with limitations regarding the amount of venom available. (AU)

FAPESP's process: 14/11108-0 - Comparative study of biomolecules composition of Bothrops jararaca and Crotalus durissus terrificus venom born and maintained at the Butantan Institute and newly arrived from nature
Grantee:Karen de Morais Zani
Support type: Regular Research Grants
FAPESP's process: 17/01890-0 - Comparative study of Crotalus durissus terrificus snake venoms born in captivity in the Laboratory of Herpetology of Butantan Institute and the national crotalic reference venom
Grantee:Anita Mitico Tanaka-Azevedo
Support type: Regular Research Grants
FAPESP's process: 12/19321-9 - Proteomic and peptidomic Characterization of Brazilian spider venoms by mass spectrometry
Grantee:Alexandre Keiji Tashima
Support type: Regular Research Grants
FAPESP's process: 16/09976-9 - Comparison of venom from Bothrops jararaca snake maintened in captivity and newly arrived from nature
Grantee:Nathália da Costa Galizio
Support type: Scholarships in Brazil - Scientific Initiation