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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The syk-coupled C-Type lectin receptors Dectin-2 and Dectin-3 are involved in Paracoccidioides brasiliensis recognition by human Plasmacytoid Dendritic cells

Texto completo
Autor(es):
Preite, Nycolas Willian [1] ; Feriotti, Claudia [1] ; de Lima, Dhemerson Souza [1] ; da Silva, Bruno Borges [1] ; Condino-Neto, Antonio [1] ; Pontillo, Alessandra [1] ; Garcia Calich, Vera Lucia [1] ; Loures, Flavio Vieira [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil
[2] Univ Ferderal Sao Paulo, Inst Ciencia & Tecnol, Sao Jose Dos Campos - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN IMMUNOLOGY; v. 9, MAR 20 2018.
Citações Web of Science: 1
Resumo

Plasmacytoid dendritic cells (pDCs), which have been extensively studied in the context of the immune response to viruses, have recently been implicated in host defense mechanisms against fungal infections. Nevertheless, the involvement of human pDCs during paracoccidioidomycosis (PCM), a fungal infection endemic to Latin America, has been scarcely studied. However, pDCs were found in the cutaneous lesions of PCM patients, and in pulmonary model of murine PCM these cells were shown to control disease severity. These findings led us to investigate the role of human pDCs in the innate phase of PCM. Moreover, considering our previous data on the engagement of diverse Toll-like receptors and C-type lectin receptors receptors in Paracoccidioides brasiliensis recognition, we decided to characterize the innate immune receptors involved in the interaction between human pDCs and yeast cells. Purified pDCs were obtained from peripheral blood mononuclear cells from healthy donors and they were stimulated with P. brasiliensis with or without blocking antibodies to innate immune receptors. Here we demonstrated that P. brasiliensis stimulation activates human pDCs that inhibit fungal growth and secrete pro-inflammatory cytokines and type I IFNs. Surprisingly, P. brasiliensis-stimulated pDCs produce mature IL-1 beta and activate caspase 1, possibly via inflammasome activation, which is a phenomenon not yet described during pDC engagement by microorganisms. Importantly, we also demonstrate that dectin-2 and dectin-3 are expressed on pDCs and appear to be involved (via Syk signaling) in the pDC-P. brasiliensis interaction. Moreover, P. brasiliensis-stimulated pDCs exhibited an efficient antigen presentation and were able to effectively activate CD4(+) and CD8(+) T cells. In conclusion, our study demonstrated for the first time that human pDCs are involved in P. brasiliensis recognition and may play an important role in the innate and adaptive immunity against this fungal pathogen. (AU)

Processo FAPESP: 17/00711-5 - Caracterização das pDCs humanas na PCM: expressão de receptores inatos e mecanismos de tolerância
Beneficiário:Nycolas Willian Preite
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 13/02396-9 - O papel do inflamassoma NLRP3 na paracoccidioidomicose pulmonar
Beneficiário:Claudia Feriotti
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/23189-0 - Modulação das respostas Treg/Th17 por ligantes de AhR na busca de procedimento imunoterápico para a paracoccidioidomicose pulmonar
Beneficiário:Vera Lucia Garcia Calich
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/04783-2 - Estudo da função das células dendríticas plasmocitóides e mielóides frente à infecção pelo fungo Paracoccidioides brasiliensis
Beneficiário:Flávio Vieira Loures
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores