IGF-1R mRNA expression is increased in obese children

Autor(es)

Ricco, Rafaela Cristina ^[1] ; Ricco, Rubens Garcia ^[1] ; Queluz, Mariangela Carletti ^[2] ; Sarti de Paula, Mariana Teresa ^[1] ; Atique, Patricia Volpon ^[1] ; Custodio, Rodrigo Jose ^[1] ; Tourinho Filho, Hugo ^[3] ; Liberatori, Jr., Raphael Del Roio ^[1] ; Martinelli, Jr., Carlos Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Paediat, Ribeirao Preto, SP - Brazil

^[2] Univ Ribeirao Preto, Med Course, Ribeirao Preto, SP - Brazil

^[3] Sch Phys Educ & Sports Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GROWTH HORMONE & IGF RESEARCH; v. 39, p. 1-5, APR 2018.
• Citações Web of Science: 2

Resumo

Objectives: Obese children are often taller than age-matched subjects. Reports on GH and IGF-I levels in obese individuals are controversial, with normal and reduced GH-IGF-I levels having been reported in this group of patients. Thus, the aim of this study was to analyse insulin-like growth factor type 1 receptor (IGF-IR) mRNA expression in obese children. Methods: Forty-seven pre-pubertal children were included in this study: 29 were obese and taller than their target height, and 18 were normal eutrophic controls. Fasting blood samples were collected for IGF-IR mRNA expression in isolated lymphocytes and serum IGF-I, ALS, IGFBP-3, and IGFBP-1 concentration analysis. Results: Relative IGF-IR gene expression (2(-Delta Delta CT)) was significantly (P = 0.025) higher in obese children (median 1.87) than in controls (1.15). Fourteen of the 29 obese subjects showed 2(-Delta Delta CT) values greater than or equal to 2, while only 2 individuals in the control group showed values above 2 (P = 0.01). Obese children showed significantly (P = 0.01) higher IGF-I concentrations than the control group (237 ng/ml and 144 ng/ml, respectively). Among obese patients, 65.5% had IGF-I values above the 75 percentile of the control group (P = 0.02). ALS concentration was significantly (P = 0.04) higher in the obese group, while IGFBP-3 levels were similar in obese and control children. IGFBP-1 concentration was lower in obese children, while insulin levels and HOMA-IR index were higher than in controls. Conclusions: The higher IGF-IR mRNA expression observed in obese children, associated with the higher IGF-I and ALS and the lower IGFBP-1 levels, suggest that the higher stature observed in these children may be due to increased IGF-I bioactivity. (AU)

• Processo FAPESP: 13/08949-0 - Avaliação da expressão do receptor tipo 1 dos fatores de crescimento insulina-símile (IGF1R) em crianças obesas
• Beneficiário: Carlos Eduardo Martinelli Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular