| Full text | |
| Author(s): |
Ricco, Rafaela Cristina
[1]
;
Ricco, Rubens Garcia
[1]
;
Queluz, Mariangela Carletti
[2]
;
Sarti de Paula, Mariana Teresa
[1]
;
Atique, Patricia Volpon
[1]
;
Custodio, Rodrigo Jose
[1]
;
Tourinho Filho, Hugo
[3]
;
Liberatori, Jr., Raphael Del Roio
[1]
;
Martinelli, Jr., Carlos Eduardo
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Paediat, Ribeirao Preto, SP - Brazil
[2] Univ Ribeirao Preto, Med Course, Ribeirao Preto, SP - Brazil
[3] Sch Phys Educ & Sports Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | GROWTH HORMONE & IGF RESEARCH; v. 39, p. 1-5, APR 2018. |
| Web of Science Citations: | 2 |
| Abstract | |
Objectives: Obese children are often taller than age-matched subjects. Reports on GH and IGF-I levels in obese individuals are controversial, with normal and reduced GH-IGF-I levels having been reported in this group of patients. Thus, the aim of this study was to analyse insulin-like growth factor type 1 receptor (IGF-IR) mRNA expression in obese children. Methods: Forty-seven pre-pubertal children were included in this study: 29 were obese and taller than their target height, and 18 were normal eutrophic controls. Fasting blood samples were collected for IGF-IR mRNA expression in isolated lymphocytes and serum IGF-I, ALS, IGFBP-3, and IGFBP-1 concentration analysis. Results: Relative IGF-IR gene expression (2(-Delta Delta CT)) was significantly (P = 0.025) higher in obese children (median 1.87) than in controls (1.15). Fourteen of the 29 obese subjects showed 2(-Delta Delta CT) values greater than or equal to 2, while only 2 individuals in the control group showed values above 2 (P = 0.01). Obese children showed significantly (P = 0.01) higher IGF-I concentrations than the control group (237 ng/ml and 144 ng/ml, respectively). Among obese patients, 65.5% had IGF-I values above the 75 percentile of the control group (P = 0.02). ALS concentration was significantly (P = 0.04) higher in the obese group, while IGFBP-3 levels were similar in obese and control children. IGFBP-1 concentration was lower in obese children, while insulin levels and HOMA-IR index were higher than in controls. Conclusions: The higher IGF-IR mRNA expression observed in obese children, associated with the higher IGF-I and ALS and the lower IGFBP-1 levels, suggest that the higher stature observed in these children may be due to increased IGF-I bioactivity. (AU) | |
| FAPESP's process: | 13/08949-0 - Insulin-like type 1 receptor (IGF1R) expression in obese children |
| Grantee: | Carlos Eduardo Martinelli Junior |
| Support Opportunities: | Regular Research Grants |