Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The glutamine synthetase of Trypanosoma cruzi is required for its resistance to ammonium accumulation and evasion of the parasitophorous vacuole during host-cell infection

Texto completo
Autor(es):
Mostrar menos -
Crispim, Marcell [1] ; Damasceno, Flavia Silva [1] ; Hernandez, Agustino [1] ; Barison, Maria Julia [1] ; Sauter, Ismael Pretto [2] ; Pavani, Raphael Souza [3] ; Moura, Alexandre Santos [1] ; Furusho Pral, Elizabeth Mieko [1] ; Cortez, Mauro [2] ; Elias, Maria Carolina [3] ; Silber, Ariel Mariano [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Lab Biochem Tryps LaBTryps, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Immunobiol Leishmania Macrophage Interact Lab, Sao Paulo - Brazil
[3] Butantan Inst, Ctr Toxins Immunol & Cell Signalling, Special Lab Cell Cycle, Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PLoS Neglected Tropical Diseases; v. 12, n. 1 JAN 2018.
Citações Web of Science: 4
Resumo

Trypanosoma cruzi, the etiological agent of Chagas disease, consumes glucose and amino acids depending on the environmental availability of each nutrient during its complex life cycle. For example, amino acids are the major energy and carbon sources in the intracellular stages of the T. cruzi parasite, but their consumption produces an accumulation of NH4+ in the environment, which is toxic. These parasites do not have a functional urea cycle to secrete excess nitrogen as low-toxicity waste. Glutamine synthetase (GS) plays a central role in regulating the carbon/nitrogen balance in the metabolism of most living organisms. We show here that the gene TcGS from T. cruzi encodes a functional glutamine synthetase; it can complement a defect in the GLN1 gene from Saccharomyces cerevisiae and utilizes ATP, glutamate and ammonium to yield glutamine in vitro. Overall, its kinetic characteristics are similar to other eukaryotic enzymes, and it is dependent on divalent cations. Its cytosolic/ mitochondrial localization was confirmed by immunofluorescence. Inhibition by Methionine sulfoximine revealed that GS activity is indispensable under excess ammonium conditions. Coincidently, its expression levels are maximal in the amastigote stage of the life cycle, when amino acids are preferably consumed, and NH4+ production is predictable. During host-cell invasion, TcGS is required for the parasite to escape from the parasitophorous vacuole, a process sine qua non for the parasite to replicate and establish infection in host cells. These results are the first to establish a link between the activity of a metabolic enzyme and the ability of a parasite to reach its intracellular niche to replicate and establish host-cell infection. (AU)

Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
Beneficiário:Hugo Aguirre Armelin
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 16/06034-2 - O papel biológico de aminoácidos e seus metabólitos derivados em Trypanosoma cruzi
Beneficiário:Ariel Mariano Silber
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?
Beneficiário:Maria Carolina Quartim Barbosa Elias Sabbaga
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/10580-0 - Caracterização de checkpoint intra-S em células de Trypanosoma
Beneficiário:Maria Carolina Quartim Barbosa Elias Sabbaga
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/10443-0 - Mecanismos e consequências do travamento do tráfego intracelular por 8- e 14-dehidroesteróis em modelos de parasitos fúngicos
Beneficiário:Agustín Hernández López
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores