Extracellular matrix dynamics during mesenchymal stem cells differentiation

Autor(es)

Assis-Ribas, Thais ^[1] ; Forni, Maria Fernanda ^[2] ; Brochado Winnischofer, Sheila Maria ^[3] ; Sogayar, Mari Cleide ^{[2, 1]} ; Trombetta-Lima, Marina ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, NUCEL NETCEM Fac Med, Dept Clin Med, BR-05360120 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531990 Curitiba, Parana - Brazil

^[4] Univ Sao Paulo, NUCEL NETCEM Fac Med, Rua Pangare, 100 Cidade Univ, BR-05360120 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Developmental Biology; v. 437, n. 2, p. 63-74, MAY 15 2018.
• Citações Web of Science: 12

Resumo

Mesenchymal stem cells (MSCs) are stromal cells that display self-renewal and multipotent differentiation capacity. The repertoire of mature cells generated ranges but is not restricted to: fat, bone and cartilage. Their potential importance for both cell therapy and maintenance of in vivo homeostasis is indisputable. Nonetheless, both their in vivo identity and use in cell therapy remain elusive. A drawback generated by this fact is that little is known about the MSC niche and how it impacts differentiation and homeostasis maintenance. Hence, the roles played by the extracellular matrix (ECM) and its main regulators namely: the Matrix Metalloproteinases (MMPs) and their counteracting inhibitors (TIMP5 and RECK) upon stem cells differentiation are only now beginning to be unveiled. Here, we will focus on mesenchymal stem cells and review the main mechanisms involved in adipo, chondro and osteogenesis, discussing how the extracellular matrix can impact not only lineage commitment, but, also, their survival and potentiality. This review critically analyzes recent work in the field in an effort towards a better understanding of the roles of Matrix Metalloproteinases and their inhibitors in the above-cited events. (AU)

• Processo FAPESP: 15/26328-8 - Análise comparativa do papel do gene supressor de tumor RECK na sub-população de células-tronco tumorais de GBM e em precursores neurais normais.
• Beneficiário: Marina Trombetta Lima
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/05311-2 - Medicina regenerativa visando à terapia de doenças crônico-degenerativas (câncer e diabetes)
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Temático
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• Beneficiário: Maria Fernanda Pereira de Araújo Demonte Forni
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/18277-7 - Análise do papel do gene supressor de tumor Reck em divisões celulares assimétricas
• Beneficiário: Marina Trombetta Lima
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 15/25776-7 - Mecanismos Moleculares do Particionamento Mitocondrial em Células de Mamífero.
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado