Extracellular matrix dynamics during mesenchymal stem cells differentiation

Author(s)

Assis-Ribas, Thais ^[1] ; Forni, Maria Fernanda ^[2] ; Brochado Winnischofer, Sheila Maria ^[3] ; Sogayar, Mari Cleide ^{[2, 1]} ; Trombetta-Lima, Marina ^{[1, 4]}

Affiliation

^[1] Univ Sao Paulo, NUCEL NETCEM Fac Med, Dept Clin Med, BR-05360120 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531990 Curitiba, Parana - Brazil

^[4] Univ Sao Paulo, NUCEL NETCEM Fac Med, Rua Pangare, 100 Cidade Univ, BR-05360120 Sao Paulo, SP - Brazil

• Document type: Review article
• Source: Developmental Biology; v. 437, n. 2, p. 63-74, MAY 15 2018.
• Web of Science Citations: 12

Abstract

Mesenchymal stem cells (MSCs) are stromal cells that display self-renewal and multipotent differentiation capacity. The repertoire of mature cells generated ranges but is not restricted to: fat, bone and cartilage. Their potential importance for both cell therapy and maintenance of in vivo homeostasis is indisputable. Nonetheless, both their in vivo identity and use in cell therapy remain elusive. A drawback generated by this fact is that little is known about the MSC niche and how it impacts differentiation and homeostasis maintenance. Hence, the roles played by the extracellular matrix (ECM) and its main regulators namely: the Matrix Metalloproteinases (MMPs) and their counteracting inhibitors (TIMP5 and RECK) upon stem cells differentiation are only now beginning to be unveiled. Here, we will focus on mesenchymal stem cells and review the main mechanisms involved in adipo, chondro and osteogenesis, discussing how the extracellular matrix can impact not only lineage commitment, but, also, their survival and potentiality. This review critically analyzes recent work in the field in an effort towards a better understanding of the roles of Matrix Metalloproteinases and their inhibitors in the above-cited events. (AU)

• FAPESP's process: 15/26328-8 - Comparative analysis of the Reck tumor suppressor gene role in glioblastoma stem cell sub-population and in normal neural stem cells.
• Grantee: Marina Trombetta Lima
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/05311-2 - Regenerative medicine aiming at therapy for chronic degenerative diseases (cancer and diabetes)
• Grantee: Mari Cleide Sogayar
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/22298-0 - Bioenergetic characterization of epidermal and dermal white adipose tissue progenitors in calorically-restricted mice: dissecting the role of mitochondrial metabolism in stem cell commitment and terminal differentiation
• Grantee: Maria Fernanda Pereira de Araújo Demonte Forni
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 16/18277-7 - ANALYSIS OF THE RECK TUMOR SUPPRESSOR GENE'S ROLE IN ASYMMETRIC CELL DIVISION
• Grantee: Marina Trombetta Lima
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 15/25776-7 - Mitochondrial Partitioning in Mammalian Cells.
• Grantee: Maria Fernanda Pereira de Araújo Demonte Forni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral