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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effects of dietary omega-3 on dystrophic cardiac and diaphragm muscles as evaluated by H-1 magnetic resonance spectroscopy: Metabolic profile and calcium-related proteins

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Autor(es):
Mauricio, Adriana Fogagnolo [1] ; de Carvalho, Samara Camacari [1] ; Santo Neto, Humberto [1] ; Marques, Maria Julia [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Dept Struct & Funct Biol, Inst Biol, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: CLINICAL NUTRITION ESPEN; v. 20, p. 60-67, AUG 2017.
Citações Web of Science: 2
Resumo

Background \& aims: Duchenne muscular dystrophy (DMD) is characterized by the absence of dystrophin and muscle degeneration. Calcium dysregulation and oxidative stress also contribute to the disease progression. We evaluated the potential therapeutic benefits of supplementation with omega-3 on the metabolic profile, calcium-related proteins and oxidative stress response in the heart and diaphragm (DIA) of the mdx mouse model of DMD at later stages of the disease (13 months). Methods: Mdx mice (8 months old) received omega-3 via a dietary supplement for 5 months. Metabolites were analyzed by H-1 magnetic resonance spectroscopy. Muscle total calcium was evaluated by inductively coupled plasma-optical emission spectrometry. Calsequestrin, TRPC1 and 4-HNE were determined via Western blot. Results: Omega-3 decreased the metabolites taurine (related to calcium regulation and oxidative stress), aspartate (related to inflammation) and oxypurinol (related to oxidative stress) in the heart (aspartate) and DIA (taurine, aspartate and oxypurinol). Omega-3 also significantly decreased total calcium and TRPC1 levels in cardiac and DIA muscles and increased the levels of calsequestrin (cardiac and skeletal) and decreased the oxidative stress marker 4-HNE. Conclusions: The current study suggests that supplementation with omega-3 may generate therapeutic benefits on dystrophy progression, at later stages of the disease, with changes in the metabolic profile that may be correlated to omega-3 therapy. (C) 2017 European Society for Clinical Nutrition and Metabolism. Published by Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/51697-6 - Mecanismos de ação do ácido eicosapentanóico (EPA) na proteção a mionecrose em fibras musculares distróficas
Beneficiário:Maria Julia Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/15492-3 - Terapia farmacológica das distrofinopatias: fibrose e regeneração muscular em camundongos mdx tratados com ômega-3
Beneficiário:Samara Camaçarí de Carvalho
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/04782-6 - Estudos pré-clínicos no camundongo mdx: metabolômica, biomarcadores e terapia com ômega-3
Beneficiário:Maria Julia Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/58491-1 - Mecanismos de proteção da distrofia muscular: estudo proteômico e terapia farmacológica
Beneficiário:Maria Julia Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/13577-1 - Biomarcadores da cardiomiopatia para distrofia muscular: estudo metabolômico e terapia farmacológica
Beneficiário:Adriana Fogagnolo Mauricio
Linha de fomento: Bolsas no Brasil - Doutorado