In-Depth Venome of the Brazilian Rattlesnake Crotalus durissus terrificus: An Integrative Approach Combining Its Venom Gland Transcriptome and Venom Proteome

Autor(es)

Wiezel, Gisele A. ^[1] ; Shibao, Priscila Y. T. ^[1] ; Cologna, Camila T. ^[1] ; Morandi Filho, Romualdo ^[2] ; Ueira-Vieira, Carlos ^[2] ; De Pauw, Edwin ^[3] ; Quinton, Loic ^[3] ; Arantes, Eliane C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Phys & Chem, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto - Brazil

^[2] Univ Fed Uberlandia, Genet Lab, Inst Biotechnol, Rua Acre S-N, BR-38400902 Uberlandia, MG - Brazil

^[3] Univ Liege, Lab Mass Spectrometry, MolSys Res Unit, Dept Chem, Bat B6c, B-4000 Liege - Belgium

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 17, n. 11, p. 3941-3958, NOV 2018.
• Citações Web of Science: 2

Resumo

Snake venoms are complex mixtures mainly composed of proteins and small peptides. Crotoxin is one of the most studied components from Crotalus venoms, but many other components are less known due to their low abundance. The venome of Crotalus durissus terrificus, the most lethal Brazilian snake, was investigated by combining its venom gland transcriptome and proteome to create a holistic database of venom compounds unraveling novel toxins. We constructed a cDNA library from C. d. terrificus venom gland using the Illumina platform and investigated its venom proteome through high resolution liquid chromotography-tandem mass spectrometry. After integrating data from both data sets, more than 30 venom components classes were identified by the transcriptomic analysis and 15 of them were detected in the venom proteome. However, few of them (PLA2, SVMP, SVSP, and VEGF) were relatively abundant. Furthermore, only seven expressed transcripts contributed to similar to 82% and similar to 73% of the abundance in the transcriptome and proteome, respectively. Additionally, novel venom proteins are reported, and we highlight the importance of using different databases to perform the data integration and discuss the structure of the venom components-related transcripts identified. Concluding, this research paves the way for novel investigations and discovery of future pharmacological agents or targets in the antivenom therapy. (AU)

• Processo FAPESP: 15/17466-8 - Plataforma Venômica: Proteoma da peçonha da formiga Pachycondyla villosa e análise integrada dos resultados obtidos através de ferramentas ômicas
• Beneficiário: Camila Takeno Cologna
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/26200-6 - Plataforma Venômica: Proteoma, Transcriptoma, Clonagem e Expressão de toxinas de interesse biotecnológico presentes na peçonha de Pachycondyla villosa
• Beneficiário: Camila Takeno Cologna
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
• Beneficiário: Suely Vilela
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/00586-6 - Caracterização de um inibidor de fosfolipase A2 da glândula de peçonha de Crotalus durissus terrificus: um possível adjuvante na terapia do envenenamento
• Beneficiário: Gisele Adriano Wiezel
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/15644-3 - Transcriptoma da glândula mucosa de Rhinella schneideri
• Beneficiário: Priscila Yumi Tanaka Shibao
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/18432-0 - Bioprospecção de toxinas animais com interesse biotecnológico a partir de ferramentas ômicas
• Beneficiário: Eliane Candiani Arantes Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular